Team:Paris Liliane Bettencourt/Notebook/2011/09/04

From 2011.igem.org

(Difference between revisions)
(Overview)
(Overview)
Line 9: Line 9:
*pHyperspank_tRNA : S38 in S82 --- in progress
*pHyperspank_tRNA : S38 in S82 --- in progress
*pHyperspank_tRNA_TT : S58 in S82 --- in progress
*pHyperspank_tRNA_TT : S58 in S82 --- in progress
-
*pT7_RBS_GFP_TT : S79
+
*pT7_RBS_GFP_TT :  
-
**currently in ampR plasmid (pSB1AK3?).
+
**S79: this construct in ampR plasmid (pSB1AK3?).
**functionality has been tested through transformation in BL21 and microscopy. (cf.27/08/11)
**functionality has been tested through transformation in BL21 and microscopy. (cf.27/08/11)

Revision as of 22:36, 3 September 2011

Danyel

Overview

The constructs involved in the tRNA project so far:

  • Pveg_spoVG_tRNA : S38 in S24 --- in progress
  • Pveg_spoVG_tRNA_TT
    • S81: this contruct in pSB1AK3 (ampR)
    • S92: this contruct in pHM3 (ampR/tetR) --- sequencing required and transformation in subtilis attempted by Adrien.
  • pHyperspank_tRNA : S38 in S82 --- in progress
  • pHyperspank_tRNA_TT : S58 in S82 --- in progress
  • pT7_RBS_GFP_TT :
    • S79: this construct in ampR plasmid (pSB1AK3?).
    • functionality has been tested through transformation in BL21 and microscopy. (cf.27/08/11)