Team:Paris Liliane Bettencourt/Notebook/2011/09/04

From 2011.igem.org

(Difference between revisions)
(Created page with "==Danyel== ===Overview=== The constructs involved in the tRNA project so far: *Pveg_spoVG_tRNA : S38 in S24 --- in progress *Pveg_spoVG_tRNA_TT **S81: this contruct in pSB1AK3 (...")
(Overview)
Line 5: Line 5:
*Pveg_spoVG_tRNA : S38 in S24 --- in progress
*Pveg_spoVG_tRNA : S38 in S24 --- in progress
*Pveg_spoVG_tRNA_TT
*Pveg_spoVG_tRNA_TT
-
**S81: this contruct in pSB1AK3 (ampR) : S58 in S24
+
**S81: this contruct in pSB1AK3 (ampR)
-
**S92: this contruct in pHM3 (ampR/tetR) : S58 in S69 --- sequencing required and transformation in subtilis attempted by Adrien.
+
**S92: this contruct in pHM3 (ampR/tetR) --- sequencing required and transformation in subtilis attempted by Adrien.
*pHyperspank_tRNA : S38 in S82 --- in progress
*pHyperspank_tRNA : S38 in S82 --- in progress
-
*pHyperspank_tRNA_TT : S38 in S82 --- in progress
+
*pHyperspank_tRNA_TT : S58 in S82 --- in progress
*pT7_RBS_GFP_TT : S79
*pT7_RBS_GFP_TT : S79
**currently in ampR plasmid (pSB1AK3?).
**currently in ampR plasmid (pSB1AK3?).
**functionality has been tested through transformation in BL21 and microscopy. (cf.27/08/11)
**functionality has been tested through transformation in BL21 and microscopy. (cf.27/08/11)

Revision as of 22:35, 3 September 2011

Danyel

Overview

The constructs involved in the tRNA project so far:

  • Pveg_spoVG_tRNA : S38 in S24 --- in progress
  • Pveg_spoVG_tRNA_TT
    • S81: this contruct in pSB1AK3 (ampR)
    • S92: this contruct in pHM3 (ampR/tetR) --- sequencing required and transformation in subtilis attempted by Adrien.
  • pHyperspank_tRNA : S38 in S82 --- in progress
  • pHyperspank_tRNA_TT : S58 in S82 --- in progress
  • pT7_RBS_GFP_TT : S79
    • currently in ampR plasmid (pSB1AK3?).
    • functionality has been tested through transformation in BL21 and microscopy. (cf.27/08/11)