Team:Paris Liliane Bettencourt/Notebook/2011/09/03/

From 2011.igem.org

(Difference between revisions)
(→Danyel)
(→Danyel)
Line 3: Line 3:
*Gel extraction of S82 digested on S & P and S82 digested on E & S
*Gel extraction of S82 digested on S & P and S82 digested on E & S
*Ligation of:  
*Ligation of:  
-
S38 in S24 (CmR)
+
**S38 in S24 (CmR)
-
S38 in S82 (CmR)
+
**S38 in S82 (CmR)
-
S58 in S82 (CmR)
+
**S58 in S82 (CmR)
-
(with negative controls for S24 and S82)
+
**(with negative controls for S24 and S82)
at ratio 6:1 and 100:1
at ratio 6:1 and 100:1

Revision as of 13:17, 3 September 2011

Danyel

  • Miniprep of S92(Pveg_spoVG_tRNA_Ter in pHM3 in MH1 cells).
  • Gel extraction of S82 digested on S & P and S82 digested on E & S
  • Ligation of:
    • S38 in S24 (CmR)
    • S38 in S82 (CmR)
    • S58 in S82 (CmR)
    • (with negative controls for S24 and S82)

at ratio 6:1 and 100:1

Retrieved from "http://2011.igem.org/Team:Paris_Liliane_Bettencourt/Notebook/2011/09/03/"