Team:Freiburg/Notebook/3 September

From 2011.igem.org

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with XbaI and PstI
with XbaI and PstI
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Incubation for 6 hours at 37°C. Vector pSB1K3 was already digested, it only needs to be digested with antarctica for one hours.
==<span style="color:blue;">blue light receptor</span>==
==<span style="color:blue;">blue light receptor</span>==

Revision as of 11:50, 3 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

3A-assembly:Digestion

Investigators: Sandra

Digestion of:

InsertI:

  • 64
  • 46

with EcorI and SpeI

InsertII:

  • 67
  • 50

with XbaI and PstI

Incubation for 6 hours at 37°C. Vector pSB1K3 was already digested, it only needs to be digested with antarctica for one hours.

blue light receptor

Stop of ligation

Investigators: Sandra

Inactivation of ligase at 80°C for 20 minutes.
Stored in the blue light box, labelled: ♥C3 Lig., and Not C3 Lig.

To do: Trafo

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

Picking clones

Investigators: Sandra

Only few colonies grew on two plates. These clones were picked and incubated in LB with amp at 37°C.

                       

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