Team:British Columbia/Modeling

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MODEL 1: Secretion and Production of Monoterpenes in Yeast

OBJECTIVE: To develop a model to assay the secretion and production of monoterpenes in yeast.

This modeling component of our project was to simulate the biochemical pathways for our genetically engineered yeasts. Simulations were programmed using the SimBiology Toolkit in MATLAB. Every yeast cell is designed to produce one specific monoterpene in mass, and others monterpenes in small amounts. Each simulation is based on the rate at which transcription, translation and monoterpene production occur. Our project is based on research that has been conducted in 2011 so some variables will need to be experimentally determined in the future to make more accurate simulations

The mevalonate pathway as found in yeast.

We are introducing gene variants for the HMG2 and ERG20 genes. We will model this simplified version of the mevalonate pathway with our mutant genes - the K6R-HMG2 and Erg20-2 genes.

This is the biochemical pathway for our simplified version of the mevalonate pathway given that GPP combines with the PgTPS-Pin2 synthase gene.

This is the biochemical pathway for our simplified version of the mevalonate pathway given that GPP combines with the PsTPS-Car1 synthase gene.

This is the biochemical pathway for our simplified version of the mevalonate pathway given that GPP combines with the PgxeTPS-Cin synthase gene.

This is the biochemical pathway for our simplified version of the mevalonate pathway given that GPP combines with the PgTPS-Cin synthase gene.

This is the biochemical pathway for our simplified version of the mevalonate pathway given that GPP combines with the PsTPS-Pin synthase gene.

MODEL 2: Dynamics of Mountain Pine Beetle Populations in British Columbia

OBJECTIVE: To construct a mathematical model that can predict the dynamics of the mountain pine beetle populations in B.C. under the influence of our synthetic product. This model can be coupled with the strategic planning for future conservation initiatives.

We will show how the pine beetle is predicted to spread in British Columbia, according to the government of British Columbia, and compare that to how we predict our modified yeasts may slow down the spread of the pine beetle.