Team:Cornell/Week 13

From 2011.igem.org

(Difference between revisions)
(→Thursday, September 1)
(→Saturday, September 3)
Line 52: Line 52:
__NOTOC__
__NOTOC__
 +
Afternoon Lab accomplished by: Jim Mathew, Charlie Chung, Nancy Li
 +
 +
Miniprep
 +
-took concentrations of yesterday miniprep
 +
-all 260/280nm ratio were precisely near 1.8 indicting pure DNA
 +
 +
DNA                                Concentration (ng/uL)
 +
RFP 1 in PSB1C3 Backbone                    344.0
 +
RFP 2 in PSB1C3 Backbone                    256.4
 +
RFP 3 in PSB1C3 Backbone                    230.4
 +
VIOA 1-1 in PSB1C3 Backbone                205.9
 +
VIOA 1-2 in PSB1C3 Backbone                494.3
 +
VIOA 1-3 in PSB1C3 Backbone                370.8
 +
VIOA 2-1 in PSB1C3 Backbone                892.5
 +
VIOA 2-2 in PSB1C3 Backbone                203.8
 +
VIOA 2-3 in PSB1C3 Backbone                206.0
 +
VIOB 1-1 in PSB1C3 Backbone                503.9
 +
VIOB 1-2 in PSB1C3 Backbone                186.1
 +
VIOB 1-3 in PSB1C3 Backbone                398.8
 +
VIOB 2-1 in PSB1C3 Backbone                868.9
 +
VIOB 2-2 in PSB1C3 Backbone                145.6
 +
VIOB 2-3 in PSB1C3 Backbone                174.6
 +
VIOE 1 in PSB1C3 Backbone                  266.2
 +
VIOE 2 in PSB1C3 Backbone                  552.9
 +
VIOE 3 in PSB1C3 Backbone                  400.7

Revision as of 20:29, 3 September 2011

Results | Protocol | Notebook | Parts Submitted

Week 1 | Week 2 - 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |


August 28th - September 3rd

Sunday, August 28

Monday, August 29

Tuesday, August 30

Afternoon - Evening lab work done by: Claire Paduano, Youjin Cho, Maneesh Gupta, James Mathew, Charlie Chung

  • Digestion Reactions
- specifics to be posted soon
  • Agarose Gel Electrophoresis
- specifics to be posted soon
  • Gel Extraction and Purification of DNA
- specifics to be posted soon (including some issues in cutting out the insert band vs. backbone band)
  • NanoDrop Spectrophometry for Purified DNA Quantification
- specific [ ]s to be posted soon
  • Ligation Reactions
- specifics to be posted soon

Wednesday, August 31

Afternoon/Evening Lab work done by: Claire Paduano and Maneesh Gupta

  • Looked at ATTO590 coated chip from Aug 27th under fluorescent microscope: channels still fluorescent
Photo to be posted soon
Streptavidin coating and Fluorescent Probes
Procedure to be posted soon
Flow experiments
Details and photos to be posted soon

Thursday, September 1

Morning lab work done by: Claire Paduano and Nancy Li

  • Coated three chips with streptavidin
Streptavidin coating
  • Protocol from Gleghorn et al: http://www.ncbi.nlm.nih.gov/pubmed/20024046
1) 45 min in 4% (by volume) MPTMS in ethanol
2) 20 min in 1mM GMBS
3) 45 min in 25ng/mL NeutrAvidin in PBS

Evening lab work done by: Maneesh Gupta

  • Did continuous flow experiments to test resilience of biotin-avitin binding
  • Used same exposure (83.33) in all images taken

Friday, September 2

Saturday, September 3

Afternoon Lab accomplished by: Jim Mathew, Charlie Chung, Nancy Li

Miniprep -took concentrations of yesterday miniprep -all 260/280nm ratio were precisely near 1.8 indicting pure DNA

DNA Concentration (ng/uL) RFP 1 in PSB1C3 Backbone 344.0 RFP 2 in PSB1C3 Backbone 256.4 RFP 3 in PSB1C3 Backbone 230.4 VIOA 1-1 in PSB1C3 Backbone 205.9 VIOA 1-2 in PSB1C3 Backbone 494.3 VIOA 1-3 in PSB1C3 Backbone 370.8 VIOA 2-1 in PSB1C3 Backbone 892.5 VIOA 2-2 in PSB1C3 Backbone 203.8 VIOA 2-3 in PSB1C3 Backbone 206.0 VIOB 1-1 in PSB1C3 Backbone 503.9 VIOB 1-2 in PSB1C3 Backbone 186.1 VIOB 1-3 in PSB1C3 Backbone 398.8 VIOB 2-1 in PSB1C3 Backbone 868.9 VIOB 2-2 in PSB1C3 Backbone 145.6 VIOB 2-3 in PSB1C3 Backbone 174.6 VIOE 1 in PSB1C3 Backbone 266.2 VIOE 2 in PSB1C3 Backbone 552.9 VIOE 3 in PSB1C3 Backbone 400.7

Retrieved from "http://2011.igem.org/Team:Cornell/Week_13"