Team:Freiburg/Notebook/2 September

From 2011.igem.org

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===Miniprep===
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'''Investigators: Sophie'''
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miniprep of GFP-pbd with IPTG-inducible promoter. The pellets were green!!! So we finally got both promoter and plastic binding domain into a vector! We can make experiments with the pbd now.
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Revision as of 16:47, 2 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

MIniprep

Investigators: Sophie, Sandra

Miniprep of new ligated parts in pSB1C3 vector.

After miniprep samples were digested and loaded onto a gel. Not c has inserts:-)

Freiburg2011 Lovtap.jpg

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

Trafo

Investigators: Julia

Trafo of ligated parts:

  1. pGEX vector (Amp. resistance)
  2. pGEX vector (Amp. resistance)
  3. pGEX vector (Amp. resistance)
  4. pSB1C3
  5. pET Duet vector (Amp. resistance)
  6. pET Duet vector (Amp. resistance)
  7. pET Duet vector (Amp. resistance)
  8. pSB1C3
  9. pSB1C3
  10. pSB1C3

Miniprep

Investigators: Sophie

miniprep of GFP-pbd with IPTG-inducible promoter. The pellets were green!!! So we finally got both promoter and plastic binding domain into a vector! We can make experiments with the pbd now.

                       

Retrieved from "http://2011.igem.org/Team:Freiburg/Notebook/2_September"