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Team:Lyon-INSA-ENS/Realisation/Week13
From 2011.igem.org
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<p style=" line-height : 1.5em"> | <p style=" line-height : 1.5em"> | ||
| + | <FONT COLOR="red"> <b>Transformations and controls for future tests</b> </FONT> <br/><br/> | ||
| - | + | ||
| + | </p> | ||
| + | |||
| + | <p style=" line-height : 1.5em"> | ||
| + | <FONT COLOR="blue"><b>Plasmid and strain Collection</b></FONT> <br/><br/> | ||
| + | Plating of S17 from the collection to extract a bigger quantity of pIG25 ( pSB1C3 containing rcn-csgBAEFG )<br/><br/> | ||
| + | |||
| + | Storage of the correct clone of:<br/> | ||
| + | PHL1414 + pIG3 (S30)<br/> | ||
| + | PHL1414+pIG16 (S31) <br/> | ||
| + | MC4100 + pIG34 (S32) <br/><br/> | ||
| + | |||
| + | Storage of rcn-OmpR in psB1K3 (pIG34) from the previous miniprep. | ||
| + | |||
| + | </p> | ||
| + | |||
| + | <p style=" line-height : 1.5em"> | ||
| + | <FONT COLOR="purple"><b> Adherence Test Preparation </b></FONT> <br/><br/> | ||
| + | |||
| + | |||
| + | </p> | ||
| + | |||
| + | <p style=" line-height : 1.5em"> | ||
| + | <FONT COLOR="orange"> <b> Others </b></FONT> <br/><br/> | ||
| + | Pouring of 9 LB+Amp plates<br/><br/> | ||
| + | |||
| + | Dilution of 5µL pIG25 (371.4 ng/µL) into 25µL water to obtain 3 additional tubes at concentration of about 50ng/µL for sequencing. <br/> | ||
| + | Sequencing (pIG25 and pIG27) sent. | ||
| + | |||
| + | </p> | ||
<br/> <br/> | <br/> <br/> | ||
Revision as of 10:45, 14 September 2011
Week 13
From Monday the 12th of September to Friday the 16th of September 2011
Monday
Transformations and controls for future tests
Plasmid and strain Collection
Plating of S17 from the collection to extract a bigger quantity of pIG25 ( pSB1C3 containing rcn-csgBAEFG )
Storage of the correct clone of:
PHL1414 + pIG3 (S30)
PHL1414+pIG16 (S31)
MC4100 + pIG34 (S32)
Storage of rcn-OmpR in psB1K3 (pIG34) from the previous miniprep.
Adherence Test Preparation
Others
Pouring of 9 LB+Amp plates
Dilution of 5µL pIG25 (371.4 ng/µL) into 25µL water to obtain 3 additional tubes at concentration of about 50ng/µL for sequencing.
Sequencing (pIG25 and pIG27) sent.
Tuesday
Wednesday
Thursday
Friday
