Team:Bilkent UNAM Turkey

From 2011.igem.org

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<p><span lang=3DTR>We aim to genetically modify unicellular microalga <i
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<p><span lang=3DTR>We aim for the genetic modification of the unicellular microalga <i
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style=3D'mso-bidi-font-style:normal'>Chlamydomonas reinhardtii</i>  by intro
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style=3D'mso-bidi-font-style:normal'>Chlamydomonas reinhardtii</i>  by introducing the
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ducing
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<i style=3D'mso-bidi-font-style:normal'>nfsI</i> gene belonging to the bacterium <i
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<i style=3D'mso-bidi-font-style:normal'>nfsI</i> gene of bacterium <i
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style=3D'mso-bidi-font-style:normal'>Enterobacter cloacae</i> in order to
style=3D'mso-bidi-font-style:normal'>Enterobacter cloacae</i> in order to
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investigate how nitroreductase expressing-microalgae respond to trinitrotol
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investigate how nitroreductase expressing-microalgae respond to trinitrotoluene
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uene
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(TNT) exposure. Our experimental design is as follows:  
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(TNT) exposure. Our experimental design is as follows: obtain a synthetic g
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1. Obtain a synthetic gene of <i style=3D'mso-bidi-font-style:normal'>nfsI</i> with flanking prefix and suffix of standard Biobricks, and ligate this insert to pRbcBRL, a vector with
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ene
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appropriate expression and selection system for <i style=3D'mso-bidi-font-style:
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of <i style=3D'mso-bidi-font-style:normal'>nfsI</i> with flanking prefix and
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normal'>Chlamydomonas reinhardtii</i> and obtain pRbcnfsI. Then, <i style=3D'mso-bidi-font-style:normal'>Chlamydomonas reinhardtii</i> will be transfected with the with the designed plasmid. The transfected algae will then be grown in presence of TNT and/or TNT derivatives and the effectiveness of nitroreductase activity on
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suffix of standard Biobricks, and ligate this insert to pRbcBRL, a vector w
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biological degradation of TNT will be investigated.<b style=3D'mso-bidi-font-weight:normal'><o:p
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ith
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appropriate expression and selection system for <i style=3D'mso-bidi-font-s
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tyle:
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normal'>Chlamydomonas reinhardtii</i> and obtain pRbcnfsI. Then, we can
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transform <i style=3D'mso-bidi-font-style:normal'>Chlamydomonas reinhardtii  
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</i>
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with designed plasmid. After engineering microalgae, we will grow them in t
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he
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presence of TNT and investigate effectiveness of nitroreductase activity on
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biological degradation of TNT.<b style=3D'mso-bidi-font-weight:normal'><o:p
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></o:p></b></span></p>
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Revision as of 21:24, 2 September 2011

 
Team Project Modelling
Deneme3 Deneme4
Lab
Notebook Lab Safety
Biobrick Parts Attributions

  • Chlamy the TermiNaTor

    Abstract

    We aim for the genetic modification of the unicellular microalga Chlamydomonas reinhardtii by introducing the nfsI gene belonging to the bacterium Enterobacter cloacae in order to investigate how nitroreductase expressing-microalgae respond to trinitrotoluene (TNT) exposure. Our experimental design is as follows: 1. Obtain a synthetic gene of nfsI with flanking prefix and suffix of standard Biobricks, and ligate this insert to pRbcBRL, a vector with appropriate expression and selection system for Chlamydomonas reinhardtii and obtain pRbcnfsI. Then, Chlamydomonas reinhardtii will be transfected with the with the designed plasmid. The transfected algae will then be grown in presence of TNT and/or TNT derivatives and the effectiveness of nitroreductase activity on biological degradation of TNT will be investigated.

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