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Team:WashU/Notebook
From 2011.igem.org
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**Recheck gene and restriction sites to make sure enzyme does not cut in the middle of the gene | **Recheck gene and restriction sites to make sure enzyme does not cut in the middle of the gene | ||
*Design Primers | *Design Primers | ||
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Revision as of 20:45, 1 June 2011
This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
You MUST have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page. PLEASE keep all of your pages within your teams namespace.
| You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing. |  |
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Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs) | File:WashU team.png Your team picture |
| Team Example |
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
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Notebook
You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.
May 31
- PCR shopping list
- PCR buffer w/o MgCl - 5ML - $45.00
- M8787- 5ML -MgCl Reagent- $49.30
- D7295-.5ML - dNTP - $73.00
- D4184-250UN - Taq Polymerase - $150.00
- D8045-250UN - AccuTaq - $300
- E1385-5ML – Ethidium Bromide – $23.70
- T8280-1L – Tris Acetate EDTA Buffer – $45.10
- NA1020-1KT – PCR Clean-Up Kit -$102.50
- NA1111-1KT - Gel Extraction Kit - $102.00
Subtotal: $890.60
- Meeting with Cohen grad students to finalize plan and get cassettes
June 1
- Lesson from Burt on how to design primers
- Codon Optimized the four genes using tool at: http://www.encorbio.com/protocols/Codon.htm
- Recheck gene and restriction sites to make sure enzyme does not cut in the middle of the gene
- Design Primers
