Team:Kyoto/Luminescence/Note

From 2011.igem.org

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(Aug 4th, 2011)
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|+ [http://www.biol.se.tmu.ac.jp/fly/www/standard-medium.html Standard feed of Tokyo Metropolitan Univ. for drosophila]
|+ [http://www.biol.se.tmu.ac.jp/fly/www/standard-medium.html Standard feed of Tokyo Metropolitan Univ. for drosophila]
! width="300" |Materials
! width="300" |Materials
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! width="300" | Making
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! width="300" |Methods
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Revision as of 06:30, 31 August 2011

This page is the note of Luminescence Group.

Aug 4th, 2011

  • We have gotten drosophila melanogaster(oregon-rs) in 3 tubes from [http://gcoe.biol.sci.kyoto-u.ac.jp/gcoe/eng/member/2008/07/fuse_naoyuki.php Mr. Fuse].
  • We have made [http://www.biol.se.tmu.ac.jp/fly/www/standard-medium.html standard feed of Tokyo Metropolitan Univ. for drosophila].
[http://www.biol.se.tmu.ac.jp/fly/www/standard-medium.html Standard feed of Tokyo Metropolitan Univ. for drosophila]
Materials Methods
  • water 500mL
  • dry yeast 20g
  • corn flour 45g
  • glucose 50g
  • agalose 3.5g
  • propionic acid 1.5mL
  1. Stir dry yeast and agalose with about two-thirds of water. Then, autoclave it.
  2. Stir corn flour and glucose with the remaining water.
  3. Stir 1 and 2, then autoclave it again.
  4. after autoclave, add propionic acid into it.■
  • We have dispensed the standard-medium into 3 bottles.