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| - | <h2>SAFETY AND SECURITY QUESTIONS</h2>
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| - | <h3>1. Would the materials used in your project and/or your final product pose:</h3>
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| - | <b>a. Risks to the safety and health of team members or others in the lab?</b>
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| - | <br>Using hazardous materials always has risk to cause trouble for team and lab members. We always <br>use hoods and wearing lab coat, gloves, goggles are essential for an experiment.
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| - | <br>We are working on two species one of them is Chlamydomonas reinhardtii which has no dangerous <br>effect on human health. Other one is Bacillus subtilis which also is not human pathogen but it <br>could cause food poisoning.
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| - | <br><b>b. Risks to the safety and health of the general public if released by design or accident?</b>
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| - | <br><i>Chlamydomonas reinhardtii is a strain of algae and it could cause algal bloom that means it <br>overgrown in a water ecosystem and become poisonous for other species which share same places.
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| - | <br>According to a Toxic Substances Control Act report from the Environmental Protection Agency, <br>Bacillus subtilis “is considered a benign organism as it does not possess traits that cause <br>disease. It is not considered pathogenic or toxigenic to humans, animals, or plants. The potential <br>risk associated with the use of this bacterium in fermentation facilities is low.” Its degree of <br>toxicity is III and IV the lowest toxicity effect on other species.
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| - | <br><b>c. Risks to environmental quality if released by design or accident?</b>
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| - | <br>We haven’t checked modified organism is more competitive than natural strains. However; Bacillus <br>subtilis is well-known and commonly used model organism like Chlamydomonas reinhardtii.
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| - | <br><b>d. Risks to security through malicious misuse by individuals, groups or states?</b>
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| - | <br><i>Our modified Chlamydomonas reinhardtii just degrade TNT and it could not use for terrorism <br>activity. Those two modified organism could not have ability to damage human health. Our facility <br>always has an active security system and it requires an identity card for enterance.
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| - | <br>Please explain your responses (whether yes or no) to these questions.
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| - | <br><b>Specifically, are any parts or devices in your project associated with (or known to cause):</i></b>
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| - | <br>- pathogenicity, infectivity, or toxicity?
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| - | <br><i>No</i>
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| - | <br>- threats to environmental quality?
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| - | <br><i>No</i>
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| - | <br>- security concerns?
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| - | <br><i>No</i>
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| - | <br><h3>2. If your response to any of the questions above is yes: </h3>
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| - | <b>a. Explain how you addressed these issues in project design and while conducting laboratory work.</b><br>
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| - | <i>Natural strain cause algal bloom and threat environmental quality so we used biological <br>hazardous waste for them. And after our work is finished, we applies bleach (%5) and then it goes <br>to waste.</i>
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| - | <br><b>b. Describe and document safety, security, health and/or environmental issues as you submit your <br>parts to the Registry.</b>
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| - | <br><i>Our parts have not any safety, security and health issues. However, adding NfsI gene into algae <br>could make it more competitive than normal strains. It requires checking before releasing to <br>nature.</i>
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| - | <br><h3>3. Under what biosafety provisions will / do you operate? </h3>
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| - | <b>a. Does your institution have its own biosafety rules and if so what are they? Provide a link to <br>them online if possible. </b>
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| - | <br><i>Our institution have its own biosafety rules and it takes 19 page so we added a link below which <br>leads to institution’s safety committee page and it contains two link; one of them orientation <br>of our lab and other one is chemical disposal form.
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| - | <br></i><a href="http://unam.bilkent.edu.tr/UNAM%20Laboratory%20Safety.html">http://unam.bilkent.edu.tr/UNAM%20Laboratory%20Safety.html</a>
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| - | <br><b>b. Does your institution have an Institutional Biosafety Committee or equivalent group? If yes, <br>have you discussed your project with them? Describe any concerns or changes that were made based <br>on this review. </b>
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| - | <br><i>We have a Laboratory Safety Committee; however, we did not discuss about our project because our <br>experiment contains standard cloning procedure, which has always done in the lab.
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| - | <br><b>c. Will / did you receive any biosafety and/or lab training before beginning your project? If <br>so, describe this training.</b>
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| - | <br><i>Our institution have an exam about biosafety, we passed it. It consists of orientation which <br>mentioned above.</i>
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| - | <br><br><b>d. Does your country have national biosafety regulations or guidelines? If so, provide a link to <br>them online if possible.</b>
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| - | <br><a href="http://www.unep.org/biosafety/files/TRNBFrep.pdf">http://www.unep.org/biosafety/files/TRNBFrep.pdf</a>
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