Team:Cambridge/Experiments/Reflectin Thin Films II

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==Results==
==Results==
The centrifugation appears to have reduced the disruption due to impurities in the coated solution  
The centrifugation appears to have reduced the disruption due to impurities in the coated solution  
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[[File:Reflectin Thin Film3.jpg |thumb|200px|left| Reflectin Thin Film made with protein/HFIP solution (not centrifuged]]
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[[File:Reflectin Thin Film3.jpg |thumb|500px|left| Reflectin Thin Film made with protein/HFIP solution (not centrifuged]]
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[[File:Reflectin Thin Film 2.jpg |thumb|200px|Reflectin Thin Film made with centrifuged protein/HFIP solution]]
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[[File:Reflectin Thin Film 2.jpg |thumb|500px|left|Reflectin Thin Film made with centrifuged protein/HFIP solution]]
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[[File:BSAcontrolfilm1.jpg |thumb|500px|left|Thin Film made with Bovine Serum Albumin/HFIP solution]]
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==Safety==
==Safety==
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{{Template:Team:Cambridge/CAM_2011_TEMPLATE_FOOT}}

Revision as of 20:52, 28 August 2011

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OVERVIEW
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Contents

Reflectin Thin Films II

We refined our methods in a number of ways:

Practice

  • Centrifugation of the solutions prior to spin and flow coating to sediment the impurities that caused thickness variations previously. (13000rpm for 10 minutes)
  • We introduced liquid CO2 pressure wash to improve cleaning of the silicon substrates
  • Trialled spin coating on a glass substrate to allow us to image the way the thin film refracts and transmits light.
  • We heat cured the thin films, 10 minutes at 80 or 120 degrees.
  • We recorded the reflectance spectra of some of the thin films
  • We ran a control using the protein bovine serum albuimin.

Results

The centrifugation appears to have reduced the disruption due to impurities in the coated solution

Reflectin Thin Film made with protein/HFIP solution (not centrifuged
Reflectin Thin Film made with centrifuged protein/HFIP solution
Thin Film made with Bovine Serum Albumin/HFIP solution

Safety