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Team:Korea U Seoul/Project
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| + | This is what we came up with. It is a pathway that utilizes luminescence system and fatty acid reduction pathway already present within ''E.coli''. However, if a beta oxidation pathway is activated through acyl-CoA ligase, we will not be able to use carboxylic acid in the desired way. Therefore, we must use fadD to block beta oxidation. | ||
| + | Based on these results, we carefully set up our research plan. | ||
=== Synthetic Fatty Acids Reduction Pathway === | === Synthetic Fatty Acids Reduction Pathway === | ||
Revision as of 12:55, 27 August 2011
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Overall project
The goal of our project is to produce alkane chains from glucose molecules. In nature, numerous biochemical pathways and enzymes exist, making life adoptable to even extreme conditions such as volcanic regions. We focused on biochemical pathways, enzymes of glucose metabolism and luminescene luciferase from Vibrio harveyi to achieve our goal. Based on glycolysis, pyruvate oxidation, enzymes coded in luciferase genes (lux operon) and FAC from cyanobacteria, glucose is turned into alkane chain of about 14 carbon atoms in length. Synthesized fuel is functionally identical to natural petroleum and can be used as bioenergy. Produced alkane chain is part of a carbon circulation cycle as it is synthesized from glucose, in vivo. The fuel is relatively environment-friendly, unlike ordinary petroleum which increases CO2 concentration in the atmosphere. Though the production of alkanes using bioblock could be not satisfied commercially, succeeding in the synthesis of alkane chains from glucose nevertheless will show another method of producing alternative energy source. Therefore, the success of this research will contribute to global effort in reducing atmospheric CO2 levels.
Project Details
We believe that there exists difficulty in producing Wax ester. Therefore, we read carefully several dissertations in order to find a compound that we can use as biofuel. One of our members came up with an idea after finding a picture(shown below) about a synthetic pathway in fluorescing bacteria.
According to the picture above, it is possible to turn Acyl-ACP into a C14 aldehyde. We can then deduce from such a result the possibility of producing a C14 alkane from glucose, utilizing microorganisms as a synthetic machinery.
By considering such possibilities, team members began searching for pathways that utilize two substances, which are: 1. luxAB removed luxCDE from fluorescing bacteria 2. cyanobacteria's aldehyde decarbonylase gene
Picture below depicts biofuel synthetic pathway.
This is what we came up with. It is a pathway that utilizes luminescence system and fatty acid reduction pathway already present within E.coli. However, if a beta oxidation pathway is activated through acyl-CoA ligase, we will not be able to use carboxylic acid in the desired way. Therefore, we must use fadD to block beta oxidation.
Based on these results, we carefully set up our research plan.
Synthetic Fatty Acids Reduction Pathway
Cloning of target genes
