Team:Tokyo-NoKoGen/Team

From 2011.igem.org

(Difference between revisions)
Line 1: Line 1:
 +
<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN" "http://www.w3.org/TR/html4/loose.dtd">
<html>
<html>
<head>
<head>
<meta http-equiv=Content-Type content="text/html; charset=ISO-8859-1">
<meta http-equiv=Content-Type content="text/html; charset=ISO-8859-1">
-
<title>TEAM</title>
+
<title>Team</title>
-
<meta name="viewport" content="width = 960, minimum-scale = 0.25, maximum-scale = 1.60">
+
<meta name="viewport" content="width = 1219, minimum-scale = 0.25, maximum-scale = 1.60">
-
<meta name="GENERATOR" content="Freeway 5 Express 5.6.0 Trial">
+
<meta name="GENERATOR" content="Freeway 5 Express 5.5.2">
<style type="text/css">
<style type="text/css">
<!--  
<!--  
Line 23: Line 24:
h1:first-child { margin-top:0px }
h1:first-child { margin-top:0px }
strong { font-weight:bold }
strong { font-weight:bold }
-
.style9 { color:#00f94f; font-family:Chalkduster; font-size:70px; text-align:center }
+
.style36 { color:#f8fa53; font-family:Arial,Helvetica,sans-serif; font-size:50px; text-align:right }
-
.style1 { text-align:center }
+
.style34 { color:#fff; font-family:Arial,Helvetica,sans-serif; font-size:12px; text-align:right }
-
.style6 { color:#000; font-family:Arial,Helvetica,sans-serif; font-size:14px }
+
.style3 { font-family:Arial,Helvetica,sans-serif; margin-left:40px; margin-right:40px }
-
.style5 { color:#97238d }
+
.style14 { color:#000; font-size:20px }
-
#fwNav1 { float:right;  padding:0; margin:0; list-style:none; }
+
.style18 { color:#000 }
-
#fwNav1 .fwNavItem { width:auto; float:left;margin:0; padding:0; border-right:1px Solid #0E0F10; border-top:0; border-bottom:0; border-left:1px Solid #647B86; position:relative; }
+
.style2 { font-size:20px }
-
#fwNav1 .fwNavItem a { display:block;color:#FFFFFF; text-decoration:none; background:url(Resources/transparent.gif) repeat; margin:0;padding:3px 10px 3px 10px; height:1%; }
+
.style10 { color:#000 }
-
#fwNav1 .fwFirstChild { border-left:0; }
+
.style19 { font-family:Arial,Helvetica,sans-serif; font-size:18px; text-align:center }
-
#fwNav1 .fwLastChild { border-right:0; }
+
.style26 { color:#000; font-family:Arial,Helvetica,sans-serif; font-size:18px; margin-left:0px; margin-right:0px; text-align:justify }
-
#fwNav1 .fwNavItem a:hover, #fwNav1 .fwNavItem span a:hover, #fwNav1 .fwNavItem a:focus { color:#437286; text-decoration:none; background:url(Resources/transparent.gif) repeat; }
+
.style21 { color:#000; font-family:Arial,Helvetica,sans-serif; font-size:18px; margin-left:0px; margin-right:0px; text-align:justify }
-
#fwNav1 .fwNavItem a:active { text-decoration:none; background:url(Resources/transparent.gif) repeat; }
+
.style20 { color:#000; font-family:Arial,Helvetica,sans-serif; font-size:18px }
-
#fwNav1 .fwNavItem a.fwCurrent, #fwNav1 .fwNavItem a.fwCurrent:hover { display:block; color:#839FAD; background:url(Resources/transparent.gif) repeat; }
+
.style30 { font-family:Arial,Helvetica,sans-serif; font-size:18px; margin-left:0px; margin-right:0px; text-align:justify }
-
* html #fwNav1 { float:right; }
+
-
* html #fwNav1 .fwNavItem a { float:left; height:1%; }
+
-->
-->
</style>
</style>
Line 46: Line 45:
<body>
<body>
<div id="PageDiv" style="position:relative; min-height:100%">
<div id="PageDiv" style="position:relative; min-height:100%">
-
<table border=0 cellspacing=0 cellpadding=0 width=896>
+
<table border=0 cellspacing=0 cellpadding=0 width=961>
<colgroup>
<colgroup>
-
<col width=37>
+
<col width=960>
-
<col width=858>
+
<col width=1>
<col width=1>
</colgroup>
</colgroup>
<tr valign=top>
<tr valign=top>
-
<td height=10 colspan=2></td>
+
<td height=113 bgcolor="#0082ef"><p class="style36"><strong>Tokyo-NokoGen 2011</strong></p>
-
<td height=10></td>
+
<p class="style34 f-lp">Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology</p>
-
</tr>
+
-
<tr valign=top>
+
-
<td height=24></td>
+
-
<td height=24>
+
-
<div style="width:858px; position:relative; z-index:1; overflow:visible">
+
-
<div class="fwNavContainer1">
+
-
<ul class="style1 f-fp f-lp" id="fwNav1">
+
-
<li class="fwFirstChild fwNavItem"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen"><span class="style6" style="text-decoration:none"><strong><span class="style5" style="text-decoration:none">HOME</span></strong></span></a></li>
+
-
<li class="fwNavItem"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/Team"><span class="style6" style="text-decoration:none"><strong><span class="style5" style="text-decoration:none">TEAM</span></strong></span></a></li>
+
-
<li class="fwNavItem"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/Project"><span class="style6" style="text-decoration:none"><strong><span class="style5" style="text-decoration:none">PROJECT</span></strong></span></a></li>
+
-
<li class="fwNavItem"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/Parts"><span class="style6" style="text-decoration:none"><strong><span class="style5" style="text-decoration:none">PARTS</span></strong></span></a></li>
+
-
<li class="fwNavItem"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/Notebook"><span class="style6" style="text-decoration:none"><strong><span class="style5" style="text-decoration:none">NOTEBOOK</span></strong></span></a></li>
+
-
<li class="fwNavItem"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/Safety"><span class="style6" style="text-decoration:none"><strong><span class="style5" style="text-decoration:none">SAFETY</span></strong></span></a></li>
+
-
<li class="fwLastChild fwNavItem"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/Sponsors"><span class="style6" style="text-decoration:none"><strong><span class="style5" style="text-decoration:none">SPONSOR</span></strong></span></a></li>
+
-
</ul>
+
-
</div>
+
-
</div>
+
</td>
</td>
-
<td height=24></td>
+
<td height=113></td>
</tr>
</tr>
<tr class="f-sp">
<tr class="f-sp">
-
<td><img src="Resources/_clear.gif" border=0 width=37 height=1 alt="" style="float:left"></td>
+
<td><img src="Resources/_clear.gif" border=0 width=960 height=1 alt="" style="float:left"></td>
-
<td><img src="Resources/_clear.gif" border=0 width=858 height=1 alt="" style="float:left"></td>
+
<td height=6><img src="Resources/_clear.gif" border=0 width=1 height=1 alt="" style="float:left"></td>
-
<td height=1><img src="Resources/_clear.gif" border=0 width=1 height=1 alt="" style="float:left"></td>
+
</tr>
</tr>
</table>
</table>
-
<table border=0 cellspacing=0 cellpadding=0 width=961>
+
<table border=0 cellspacing=0 cellpadding=0 width=963>
<colgroup>
<colgroup>
-
<col width=960>
+
<col width=6>
 +
<col width=239>
 +
<col width=11>
 +
<col width=350>
 +
<col width=355>
 +
<col width=1>
<col width=1>
<col width=1>
</colgroup>
</colgroup>
<tr valign=top>
<tr valign=top>
-
<td height=126 bgcolor="#ffffff"><p class="style9 f-lp">Tokyo-NokoGen 2011</p>
+
<td height=357 rowspan=4></td>
 +
<td height=357 rowspan=4 bgcolor="#cdcdcd"><p class="style3">&nbsp;</p>
 +
<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen"><span class="style18" style="text-decoration:underline">Home</span></a></span></strong></p>
 +
<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/Team"><span class="style18" style="text-decoration:underline">Team</span></a></span></strong></p>
 +
<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/memebers"><span class="style18" style="text-decoration:underline">Members</span></a></span></strong></p>
 +
<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/Project"><span class="style18" style="text-decoration:underline">Project: EcoLion</span></a></span></strong></p>
 +
<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/Parts"><span class="style18" style="text-decoration:underline">BioBricks</span></a></span></strong></p>
 +
<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/notebook"><span class="style18" style="text-decoration:underline">Notebook</span></a></span></strong></p>
 +
<p class="style3"><strong><span class="style14"><a href="Protocols"><span class="style18" style="text-decoration:underline">Protocols</span></a></span></strong></p>
 +
<p class="style3"><strong><span class="style14"><a href="attribution"><span class="style18" style="text-decoration:underline">Attribution</span></a></span></strong></p>
 +
<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/safety"><span class="style18" style="text-decoration:underline">Safety</span></a></span></strong></p>
 +
<p class="style3 f-lp"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/sponsors"><span class="style18" style="text-decoration:underline">Sponsors</span></a></span></strong></p>
</td>
</td>
-
<td height=126></td>
+
<td height=1 colspan=4></td>
 +
<td height=1></td>
</tr>
</tr>
-
<tr class="f-sp">
+
<tr valign=top>
-
<td><img src="Resources/_clear.gif" border=0 width=960 height=1 alt="" style="float:left"></td>
+
<td height=28 colspan=2></td>
-
<td height=25><img src="Resources/_clear.gif" border=0 width=1 height=1 alt="" style="float:left"></td>
+
<td height=26 style="border:solid #000 1px"><p class="style19 f-lp"><a href="https://igem.org/Team.cgi?id=519">Tokyo-NoKoGen 2011 official team profile</a></p>
 +
</td>
 +
<td height=28></td>
 +
<td height=28></td>
</tr>
</tr>
-
</table>
 
-
<table border=0 cellspacing=0 cellpadding=0 width=682>
 
-
<colgroup>
 
-
<col width=170>
 
-
<col width=159>
 
-
<col width=241>
 
-
<col width=111>
 
-
<col width=1>
 
-
</colgroup>
 
<tr valign=top>
<tr valign=top>
-
<td height=320 colspan=2></td>
+
<td height=5 colspan=4></td>
-
<td height=320><img src="https://static.igem.org/mediawiki/2011/5/56/Nasa.jpg" border=0 width=241 height=320 alt="nasa" style="float:left"></td>
+
<td height=5></td>
-
<td height=320></td>
+
-
<td height=320></td>
+
</tr>
</tr>
<tr valign=top>
<tr valign=top>
-
<td height=18></td>
+
<td height=323></td>
-
<td height=18 colspan=3><p class="f-lp">Nasa is trying wiki costruction.</p>
+
<td height=553 colspan=3 rowspan=2><p class="style26"> We are the Tokyo-NoKoGen 2011, a Japanese team from Tokyo University of Agriculture and Technology. 10 undergraduates and 2 graduates have been joining the project in this year. All members belong to the Department of Biotechnology and Life Science of TUA&#26481;&#20140;&#36786;&#24037;&#22823;&#23398;), in Japanese. </p>
 +
<p class="style21"><span class="style20"> </span>Tokyo-NoKoGen has participated in iGEM since 2009, making our broad knowlegde of biotechnology to use in synthetic biology. NoKoGen had worked on the <em>E. coli</em> auto protein synthesizer designated &quot;ESCAPES&quot; in 2009 and the EcoTanker in 2010. With contribusions for iGEM and synthetic biology, our teams got a bronze and a gold medal in 2009 and 2010, respectively.</p>
 +
<p class="style21"> In this year, Tokyo-NoKoGen 2011 produce EcoLion, the <em>E. coli</em> collecting heavy metal ions. The EcoLion works for bioremediation and recovery of heavy metals such as cadmium and arsenic. The EcoLion can be differenciated from other team's previous projects aiming at collecting heavy metals using <em>E. coli</em>. Our EcoLion transport heavy metal ions into its cell and capture the ions using metallothionein. Then the captured heavy metals are transferred into a bacterial microcompartment (BMC) and concentrated in it. BMCs are very atractive protein structures that function for localization particular small molesules and proteins, especially enzymes. The combination of a BMC and a metallothionein fused to locallization-tag peptide would make it possible to accumulate more amount of heavy metals in E. coli and concetrate the collected metals, thus you can collect much more heavy metals using the EcoLion.</p>
 +
<p class="style30 f-lp"> In the same time of having fun for our iGEM projects, we had many discussions on the synthetic biology and biosafety. We hope our EcoLion project contribute to expand the potential of synthetic biology for the future. </p>
</td>
</td>
-
<td height=18></td>
+
<td height=323></td>
 +
</tr>
 +
<tr valign=top>
 +
<td height=230 colspan=3></td>
 +
<td height=230></td>
</tr>
</tr>
<tr class="f-sp">
<tr class="f-sp">
-
<td><img src="Resources/_clear.gif" border=0 width=170 height=1 alt="" style="float:left"></td>
+
<td><img src="Resources/_clear.gif" border=0 width=6 height=1 alt="" style="float:left"></td>
-
<td><img src="Resources/_clear.gif" border=0 width=159 height=1 alt="" style="float:left"></td>
+
<td><img src="Resources/_clear.gif" border=0 width=239 height=1 alt="" style="float:left"></td>
-
<td><img src="Resources/_clear.gif" border=0 width=241 height=1 alt="" style="float:left"></td>
+
<td><img src="Resources/_clear.gif" border=0 width=11 height=1 alt="" style="float:left"></td>
-
<td><img src="Resources/_clear.gif" border=0 width=111 height=1 alt="" style="float:left"></td>
+
<td><img src="Resources/_clear.gif" border=0 width=350 height=1 alt="" style="float:left"></td>
 +
<td><img src="Resources/_clear.gif" border=0 width=355 height=1 alt="" style="float:left"></td>
 +
<td><img src="Resources/_clear.gif" border=0 width=1 height=1 alt="" style="float:left"></td>
<td height=1><img src="Resources/_clear.gif" border=0 width=1 height=1 alt="" style="float:left"></td>
<td height=1><img src="Resources/_clear.gif" border=0 width=1 height=1 alt="" style="float:left"></td>
</tr>
</tr>
Line 127: Line 125:
</body>
</body>
</html>
</html>
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
'''Advisor:'''
 
-
 
-
*''' Advisor''': Prof. Koji Sode
 
-
 
-
 
-
'''Instructors:'''
 
-
 
-
*'''Instructor 1''':  Assistant Prof. Ferri Stefano
 
-
*'''Instructor 2''':  Prof. Kazunori Ikebukuro
 
-
*'''Instructor 3''':  Associate Prof. Wakako Tsugawa
 
-
*'''Instructor 4''':  Kaori Tsukakoshi
 
-
*'''Instructor 5''':  Mitsuharu Nakajima
 
-
 
-
 
-
'''Undergrads:'''
 
-
 
-
*'''Student 1''':    Nasa Savory
 
-
*'''Student 2''':    Araki Masataka
 
-
*'''Student 3''':    Yuki Uchikura
 
-
*'''Student 4''':    Shoko Saito
 
-
*'''Student 5''':    Sumida Koichi
 
-
*'''Student 6''':    Saki Nakashima
 
-
*'''Student 7''':    Kotone Miyake
 
-
*'''Student 8''':    Chifuku Mita
 
-
*'''Student 9''':    Eri Kamio
 
-
*'''Student 10''':    Hikaru Sekiguchi
 
-
 
-
 
-
 
-
 
-
<gallery>
 
-
Image:Tokyo-NoKoGen_Team_member_1.png|Team member 1
 
-
Image:Tokyo-NoKoGen_Team_member_2.png|Team member 2
 
-
Image:Tokyo-NoKoGen_Team_member_3.png|Team member 3
 
-
Image:Tokyo-NoKoGen_Team_member_4.png|Team member 4
 
-
Image:Tokyo-NoKoGen_Team_member_5.png|Team member 5
 
-
Image:Tokyo-NoKoGen_Team_member_6.png|Team member 6
 
-
Image:Tokyo-NoKoGen_Team_member_7.png|Team member 7
 
-
</gallery>
 
-
 
-
 
-
== '''What we did''' ==
 
-
 
-
(Provide proper attribution for all work)
 
-
 
-
== Headline text ==
 
-
 
-
<nowiki>
 
-
[[File:test110615.jpg|200px|thumb|left|enter an explaination]]
 
-
</nowiki>
 
-
 
-
== '''Where we're from''' ==
 

Revision as of 11:07, 24 September 2011

<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN" "http://www.w3.org/TR/html4/loose.dtd"> Team

Tokyo-NokoGen 2011

Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology

 

Home

Team

Members

Project: EcoLion

BioBricks

Notebook

Protocols

Attribution

Safety

Sponsors

Tokyo-NoKoGen 2011 official team profile

We are the Tokyo-NoKoGen 2011, a Japanese team from Tokyo University of Agriculture and Technology. 10 undergraduates and 2 graduates have been joining the project in this year. All members belong to the Department of Biotechnology and Life Science of TUA東京農工大学), in Japanese.

Tokyo-NoKoGen has participated in iGEM since 2009, making our broad knowlegde of biotechnology to use in synthetic biology. NoKoGen had worked on the E. coli auto protein synthesizer designated "ESCAPES" in 2009 and the EcoTanker in 2010. With contribusions for iGEM and synthetic biology, our teams got a bronze and a gold medal in 2009 and 2010, respectively.

In this year, Tokyo-NoKoGen 2011 produce EcoLion, the E. coli collecting heavy metal ions. The EcoLion works for bioremediation and recovery of heavy metals such as cadmium and arsenic. The EcoLion can be differenciated from other team's previous projects aiming at collecting heavy metals using E. coli. Our EcoLion transport heavy metal ions into its cell and capture the ions using metallothionein. Then the captured heavy metals are transferred into a bacterial microcompartment (BMC) and concentrated in it. BMCs are very atractive protein structures that function for localization particular small molesules and proteins, especially enzymes. The combination of a BMC and a metallothionein fused to locallization-tag peptide would make it possible to accumulate more amount of heavy metals in E. coli and concetrate the collected metals, thus you can collect much more heavy metals using the EcoLion.

In the same time of having fun for our iGEM projects, we had many discussions on the synthetic biology and biosafety. We hope our EcoLion project contribute to expand the potential of synthetic biology for the future.