Team:Freiburg/Notebook/26 August

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Meeting

attendants:
Time:

green light receptor

already done:

To-do:

blue light receptor

already done:

To-do:

red light receptor

already done:

To-do:

Lysis cassette

already done:

To-do:

Precipitator

already done:

To-do:

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

blue light receptor

Transformation

Investigators: Sophie Date: 26.08.11 Continue from Date: 26.08.11 Name: Sophie Experiment Ligation Project Name: Blue light receptor

Procedure

1. take cells from -80°C freezer and put them on ice! (every eppi contains about 400 μl cells) 2. thaw cells on ice 20 minutes 3. pipette 50 μl cells and 2 μl DNA into eppi still on ice! 4. Incubate for 30 minutes on ice 5. Heat at 42°C for 60 sec 6. Incubate on ice for 5 minutes 7. Add 200 μl LB Broth 8. Incubate for 2 hours at 37°C (cells with lysis cassette at 30°C!!) 9. Plate 50 μl and 200μl on two different LB/Agar plates with appropriate antibiotic resistance

Documentation: Why are you doing this experiment? Name of the sample? Where are they stored? Name the vector with inserts, antibiotika resistance etc. Why? The last cloning was done with a NOT-PCR-Product which might make problems because it does not have enough bases after the restriciton sites.

Name of the samples: Not 1:1, Not 1:3, nOt 1:1 nOt 1:3, noT 1:1, noT 1: 3

stored in incubator on Amp plates

vector: psb1A3
inserts: Not/ nOt/ noT + LOV-3A-PCR

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME

Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME