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Team:UNICAMP-EMSE Brazil/Notebook/25 August 2011
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'''Objective:''' | '''Objective:''' | ||
:*Checking “RBS+TolC+T” and “RBS+SoxR+T” purified by Mini-prep and digested with E-P. | :*Checking “RBS+TolC+T” and “RBS+SoxR+T” purified by Mini-prep and digested with E-P. | ||
| + | [[Image:UNICAMP-EMSE Brazil Gel2 25 08 11.jpg|thumb|right|300px|<font color: blue>'''Electrophoresis Gel 2, 25/08/2011.'''</font> <br>RBS+SoxR+T C1 (d)/ RBS+SoxR+T C1 (nd)/ RBS+SoxR+T C2 (d)/ RBS+SoxR+T C2 (nd)/ RBS+SoxR+T C3 (d)/ RBS+SoxR+T C3 (nd)/ RBS+SoxR+T C4 (d)/ RBS+SoxR+T C4 (nd).]] | ||
| + | |||
*'''Ladder:''' | *'''Ladder:''' | ||
:*Bio-Rad 100bp – 10.000bp | :*Bio-Rad 100bp – 10.000bp | ||
Revision as of 12:14, 25 August 2011
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Notebook
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Gel 1:RBS+TolC+T C1 (d)/ RBS+TolC+T C1 (nd)/ RBS+TolC+T C2 (d)/ RBS+TolC+T C2 (nd)/ RBS+TolC+T C3 (d)/ RBS+TolC+T C3 (nd)/ RBS+TolC+T C4 (d)/ RBS+TolC+T C4 (nd).
25 August 2011
RBS+TolC+T and RBS+SocR_T
Objective:
- Checking “RBS+TolC+T” and “RBS+SoxR+T” purified by Mini-prep and digested with E-P.
RBS+SoxR+T C1 (d)/ RBS+SoxR+T C1 (nd)/ RBS+SoxR+T C2 (d)/ RBS+SoxR+T C2 (nd)/ RBS+SoxR+T C3 (d)/ RBS+SoxR+T C3 (nd)/ RBS+SoxR+T C4 (d)/ RBS+SoxR+T C4 (nd).
- Ladder:
- Bio-Rad 100bp – 10.000bp
- Samples:
- Gel 1: RBS+TolC+T C1 (d)/ RBS+TolC+T C1 (nd)/ RBS+TolC+T C2 (d)/ RBS+TolC+T C2 (nd)/ RBS+TolC+T C3 (d)/ RBS+TolC+T C3 (nd)/ RBS+TolC+T C4 (d)/ RBS+TolC+T C4 (nd)
- Gel 2: RBS+SoxR+T C1 (d)/ RBS+SoxR+T C1 (nd)/ RBS+SoxR+T C2 (d)/ RBS+SoxR+T C2 (nd)/ RBS+SoxR+T C3 (d)/ RBS+SoxR+T C3 (nd)/ RBS+SoxR+T C4 (d)/ RBS+SoxR+T C4 (nd)
OBS:
- D=digested
- Nd= non-digested
- Results:
| Gene | Total size (gene+vector) | Linear vector size | Gene size | Result |
|---|---|---|---|---|
| RBS+TolC+T | ~4880 pb | 3300 pb | 1675 pb | OK: complete digestion |
| RBS+SoxR+T | ~3823 pb | 3300 pb | 618 pb | Only C4 did not digested. C1 and C3 has a gene fragment near 500 pb and C2 has one near 700 bp. Wich one is the correct? |
