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Team:Michigan/Protocols
From 2011.igem.org
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| - | ''' | + | '''Basic protocols''' ::: These protocols are necessary for all lab operations. As many of the subjects are general, the protocols may contain few specific steps. |
[[Media: Autoclaving100.pdf | Autoclave usage]] | [[Media: Autoclaving100.pdf | Autoclave usage]] | ||
[[Media: OD600_100.pdf | OD600 optimization]] | [[Media: OD600_100.pdf | OD600 optimization]] | ||
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| + | [[Media: Electrophoresis.pdf | Gel electrophoresis]] | ||
Revision as of 20:53, 28 May 2011
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Protocols
Basic protocols ::: These protocols are necessary for all lab operations. As many of the subjects are general, the protocols may contain few specific steps.
Transformation ::: These protocols contain information on the production, usage, and transformation of competent cells.
Production of electrocompetent cells
Electroporation of electrocompetent cells
Production of standard competent cells
Quick Transformation (QT) of standard competent cells
Enzyme usage ::: These protocols contain information on digestion, ligation, and specific information on enzymatic operations.
