Team:Freiburg/Notebook/24 August

From 2011.igem.org

(Difference between revisions)
(green light receptor)
(PCR)
Line 105: Line 105:
What program do you use?
What program do you use?
-
20 cycles with an annealing temperature of 65/68/71 ºC (gradient-PCR) then 10 cycles with an annealing temperature of 65/70/75 ºC
+
20 cycles with an annealing temperature of 65/68/71 ºC (gradient-PCR) and touchdown then 10 cycles with an annealing temperature of 65/70/75 ºC and touchdown
 +
<br/>
 +
digested with DpnI

Revision as of 14:00, 24 August 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!