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Team:Michigan/Protocols
From 2011.igem.org
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[[Media: Autoclaving100.pdf | Autoclave usage]] | [[Media: Autoclaving100.pdf | Autoclave usage]] | ||
| + | [[Media: OD600_100.pdf | OD600 optimization]] | ||
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| + | '''Transformation''' ::: These protocols contain information on the production, usage, and transformation of competent cells. | ||
[[Media: electrocompetent.pdf | Production of electrocompetent cells]] | [[Media: electrocompetent.pdf | Production of electrocompetent cells]] | ||
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[[Media: quick_transformation.pdf | Quick Transformation (QT) of standard competent cells]] | [[Media: quick_transformation.pdf | Quick Transformation (QT) of standard competent cells]] | ||
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| + | '''Enzyme usage''' ::: These protocols contain information on digestion, ligation, and specific information on enzymatic operations. | ||
Revision as of 17:27, 26 May 2011
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All protocols used should be aggregated here.
Protocols
Fundamental protocols ::: These protocols are necessary for all lab operations. As many of the subjects are general, the protocols may contain few specific steps.
Transformation ::: These protocols contain information on the production, usage, and transformation of competent cells.
Production of electrocompetent cells
Electroporation of electrocompetent cells
Production of standard competent cells
Quick Transformation (QT) of standard competent cells
Enzyme usage ::: These protocols contain information on digestion, ligation, and specific information on enzymatic operations.
