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Team:KULeuven/Figures
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<h3>FIGURES</h3> | <h3>FIGURES</h3> | ||
| - | <h2>Figure 1</h2> | + | <a name="1"><h2>Figure 1</h2></a><br> |
| - | <img src="http://www.shbts.nl/igem/images/notebook/wetlab_fig01.jpg" border="0" | + | <img src="http://www.shbts.nl/igem/images/notebook/wetlab_fig01.jpg" border="0"><br><br> |
| - | Miniprep 13/07: L, A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, D= Ribolock-Coilicin E2 Dnase, E= ribolock3c, F= ribokey3d, G= GFP Generator, H= CrtEBI, I= CI repressor, J= ribosome binding site, K<sub>1</sub>= terminator, K<sub>2</sub>= pconst-RBS-MelA, Z= CspA Promoter, 1= luxI, 2= luxR , 3= Mnt repressor, 4= OmpA, 5= TEV cleavage site, L | + | Miniprep 13/07: L, A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, D= Ribolock-Coilicin E2 Dnase, E= ribolock3c, F= ribokey3d, G= GFP Generator, H= CrtEBI, I= CI repressor, J= ribosome binding site, K<sub>1</sub>= terminator, K<sub>2</sub>= pconst-RBS-MelA, Z= CspA Promoter, 1= luxI, 2= luxR , 3= Mnt repressor, 4= OmpA, 5= TEV cleavage site, L |
| - | <h2>Figure 2</h2> | + | <br><br><a href="javascript: history.go(-1)"><u>Click here to go back</u></a><br><br><br> |
| + | |||
| + | |||
| + | <a name="2"><h2>Figure 2</h2></a><br> | ||
| + | |||
| + | <img src="http://www.shbts.nl/igem/images/notebook/wetlab_fig02.jpg" border="0"><br><br> | ||
| + | |||
| + | Miniprep 15/07: labelling is the same as in Fig.1 except for K= pconst-RBS-MelA and Y= terminator. | ||
| + | |||
| + | <br><br><a href="javascript: history.go(-1)"><u>Click here to go back</u></a><br><br><br> | ||
| + | |||
| + | |||
| + | <a name="3"><h2>Figure 3</h2></a><br> | ||
| + | |||
| + | <img src="http://www.shbts.nl/igem/images/notebook/wetlab_fig03.jpg" border="0"><br><br> | ||
| + | |||
| + | Restriction 18/07: A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, I= CI repressor, G= GFP generator, H= CrtEBI, 3= Mnt repressor. | ||
| + | |||
| + | <br><br><a href="javascript: history.go(-1)"><u>Click here to go back</u></a><br><br><br> | ||
| + | |||
| + | |||
| + | <a name="4"><h2>Figure 4</h2></a><br> | ||
| + | |||
| + | Restriction 20/07: A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, <i>H</i><sub>2</sub>= RBS-CrtEBI, K= terminator, Z= cspA Promoter, 3<sub>1</sub>= Mnt repressor were all cut with EcoRI and SpeI. V= luxI, K= terminator, 3<sub>1</sub>= Mnt repressor, <i>H</i><sub>1</sub>= RBS-CrtEBI and G= GFP generator were all cut with XbaI and PstI. | ||
| + | |||
| + | <br><br><a href="javascript: history.go(-1)"><u>Click here to go back</u></a><br><br><br> | ||
| + | |||
| + | |||
| + | <a name="5"><h2>Figure 5</h2></a><br> | ||
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| + | Miniprep 21/07: A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, G= GFP generator, H= CrtEBI, I= CI repressor, U= constitutive promotor, V= LuxI, W= BAD promotor, X= Ribokey3d, 3= Mnt repressor. | ||
| + | |||
| + | <br><br><a href="javascript: history.go(-1)"><u>Click here to go back</u></a><br><br><br> | ||
| + | |||
| + | |||
| + | <a name="6"><h2>Figure 6</h2></a><br> | ||
| + | |||
| + | Restriction 25/07: U= Constitutieve promotor (PstI and SpeI), W= BAD promoter (PstI and SpeI), Z= CspA promotor (PstI and SpeI) K= Terminator (EcoRI and XbaI), X= ribokey3d (EcoRI and XbaI). | ||
| + | |||
| + | <br><br><a href="javascript: history.go(-1)"><u>Click here to go back</u></a><br><br><br> | ||
</div> | </div> | ||
Revision as of 12:56, 23 August 2011
FIGURES
Figure 1
Miniprep 13/07: L, A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, D= Ribolock-Coilicin E2 Dnase, E= ribolock3c, F= ribokey3d, G= GFP Generator, H= CrtEBI, I= CI repressor, J= ribosome binding site, K1= terminator, K2= pconst-RBS-MelA, Z= CspA Promoter, 1= luxI, 2= luxR , 3= Mnt repressor, 4= OmpA, 5= TEV cleavage site, L
Click here to go back
Figure 2
Miniprep 15/07: labelling is the same as in Fig.1 except for K= pconst-RBS-MelA and Y= terminator.
Click here to go back
Figure 3
Restriction 18/07: A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, I= CI repressor, G= GFP generator, H= CrtEBI, 3= Mnt repressor.
Click here to go back
Figure 4
Restriction 20/07: A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, H2= RBS-CrtEBI, K= terminator, Z= cspA Promoter, 31= Mnt repressor were all cut with EcoRI and SpeI. V= luxI, K= terminator, 31= Mnt repressor, H1= RBS-CrtEBI and G= GFP generator were all cut with XbaI and PstI.
Click here to go back
Figure 5
Miniprep 21/07: A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, G= GFP generator, H= CrtEBI, I= CI repressor, U= constitutive promotor, V= LuxI, W= BAD promotor, X= Ribokey3d, 3= Mnt repressor.
Click here to go back
Figure 6
Restriction 25/07: U= Constitutieve promotor (PstI and SpeI), W= BAD promoter (PstI and SpeI), Z= CspA promotor (PstI and SpeI) K= Terminator (EcoRI and XbaI), X= ribokey3d (EcoRI and XbaI).
Click here to go back
