Team:NTNU Trondheim/Stress Sensor

From 2011.igem.org

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The promotor is a variant of BBa_K112118, that we have made ourselves using PCR.  
The promotor is a variant of BBa_K112118, that we have made ourselves using PCR.  
The stress sensor has a total length of 2653 bp, and is located inside the plasmid pSB1A2, carrying ampicillin resistance.
The stress sensor has a total length of 2653 bp, and is located inside the plasmid pSB1A2, carrying ampicillin resistance.
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We have put the stress sensor through several tests (see figure)
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We have put the stress sensor through several tests (see [https://2011.igem.org/Team:NTNU_Trondheim/rrnB+LacI+pLac+mCherry characterization])
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[[File:Stress-sensor_rosa.png]]
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We have discovered that growing the cells containing the biosensor in a medium low in amino acids is the main way to activate it. As the figure shows, growing the cells in temperatures higher than 37 °C has little effect compared to growing them in a minimal medium.
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Already as we started this project we knew that lack of amino acids was the main trigger of ppGpp. Therefore, our results seems to indicate that our stress sensor work the way it is supposed to.
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Revision as of 08:46, 24 August 2011



Stress sensor (rrnB+LacI+pLac+mCherry)

RrnB+LacI+pLac+mCherry rosa.png


The stress sensor is our main construct. It is made from the following biobricks:

Biobrick Part number
LacI with RBS BBa_J24679
Terminator BBa_B0015
pLac BBa_R0011
mCherry with RBS and terminator BBa_J06702

The promotor is a variant of BBa_K112118, that we have made ourselves using PCR. The stress sensor has a total length of 2653 bp, and is located inside the plasmid pSB1A2, carrying ampicillin resistance. We have put the stress sensor through several tests (see characterization)

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