Team:UNICAMP-EMSE Brazil/Notebook/20 August 2011
From 2011.igem.org
(Difference between revisions)
Neshich.iap (Talk | contribs) |
Neshich.iap (Talk | contribs) |
||
Line 3: | Line 3: | ||
==20 August 2011== | ==20 August 2011== | ||
===Everybody:=== | ===Everybody:=== | ||
- | [[Image:UNICAMP-EMSE_Brazil_Gel_20_08_11.jpg|thumb|right|alt=Gel 1: flHDC1 (nd)/ flHDC1 (d)/ flHDC2 (nd)/ flHDC2 (d)/RBSTolC3 (nd)/ RBSTolC3(d)/ RBSTolC4 (nd)/ RBSTolC4 (d)/RBSGFP1 (nd)/RBSGFP1 (D)/ RBSGFP 3(ND)/RBSGFP 3 (D)/ RBSQSEBRBSQSEC 3 (ND)/ RBSQSEBRBSQSEC 3 (D).<br>Gel 2:''' / RBSQSEBRBSQSEC 4 (ND)/ RBSQSEBRBSQSEC 4 (D)/STRONG PROMOTOR 3 (ND)/ STRONG PROMOTOR 3 (D)/ STRONG PROMOTOR 4 (ND)/ STRONG PROMOTOR 4 (D)/ SOX S 3 (ND)/ SOX S 3 (D)/ SOX S 4 (ND)/SOX S 4 (D)|Electrophoresis Gel, 20/08/2011.]] | + | [[Image:UNICAMP-EMSE_Brazil_Gel_20_08_11.jpg|thumb|right|265px|alt=Gel 1: flHDC1 (nd)/ flHDC1 (d)/ flHDC2 (nd)/ flHDC2 (d)/RBSTolC3 (nd)/ RBSTolC3(d)/ RBSTolC4 (nd)/ RBSTolC4 (d)/RBSGFP1 (nd)/RBSGFP1 (D)/ RBSGFP 3(ND)/RBSGFP 3 (D)/ RBSQSEBRBSQSEC 3 (ND)/ RBSQSEBRBSQSEC 3 (D).<br> |
+ | |||
+ | Gel 2:''' / RBSQSEBRBSQSEC 4 (ND)/ RBSQSEBRBSQSEC 4 (D)/STRONG PROMOTOR 3 (ND)/ STRONG PROMOTOR 3 (D)/ STRONG PROMOTOR 4 (ND)/ STRONG PROMOTOR 4 (D)/ SOX S 3 (ND)/ SOX S 3 (D)/ SOX S 4 (ND)/SOX S 4 (D)|Electrophoresis Gel, 20/08/2011.]] | ||
'''Objective:''' Check the size of the synthetic DNA sequences (that didn´t work on 19/08/2011). | '''Objective:''' Check the size of the synthetic DNA sequences (that didn´t work on 19/08/2011). | ||
*Agarose: 0,8% | *Agarose: 0,8% |
Revision as of 23:28, 22 August 2011
Home | Project | Methods | Results | Data | Team | Notebook | Human Practices | Safety | Profile | Sponsors | Wix |
Notebook
Click on a date to see what we have done!
|
|
|
|
|
|
20 August 2011
Everybody:
Objective: Check the size of the synthetic DNA sequences (that didn´t work on 19/08/2011).
- Agarose: 0,8%
- Ladder: 100-10.000 bp (Fermentas)
- Picture legend: Upper part:
- Gel 1: flHDC1 (nd)/ flHDC1 (d)/ flHDC2 (nd)/ flHDC2 (d)/RBSTolC3 (nd)/ RBSTolC3(d)/ RBSTolC4 (nd)/ RBSTolC4 (d)/RBSGFP1 (nd)/RBSGFP1 (D)/ RBSGFP 3(ND)/RBSGFP 3 (D)/ RBSQSEBRBSQSEC 3 (ND)/ RBSQSEBRBSQSEC 3 (D)
- Gel 2: / RBSQSEBRBSQSEC 4 (ND)/ RBSQSEBRBSQSEC 4 (D)/STRONG PROMOTOR 3 (ND)/ STRONG PROMOTOR 3 (D)/ STRONG PROMOTOR 4 (ND)/ STRONG PROMOTOR 4 (D)/ SOX S 3 (ND)/ SOX S 3 (D)/ SOX S 4 (ND)/SOX S 4 (D)
Nd: non digested
D: digested
D: digested