"
Team:Lyon-INSA-ENS/Realisation/Protocols/FermentasFr
From 2011.igem.org
(Difference between revisions)
(Created page with "{{Lyon-INSA-ENS/header}} {{Lyon-INSA-ENS/blocStyle}} {{INSA-Lyon/styletestaurelie}} {{Lyon-INSA-ENS/menuhorizontalRealisation}} {{Lyon-INSA-ENS/menuRealisationProtocoles}} <html...") |
|||
| Line 2: | Line 2: | ||
{{Lyon-INSA-ENS/blocStyle}} | {{Lyon-INSA-ENS/blocStyle}} | ||
{{INSA-Lyon/styletestaurelie}} | {{INSA-Lyon/styletestaurelie}} | ||
| - | {{Lyon-INSA-ENS/ | + | {{Lyon-INSA-ENS/menuhorizontalFrReal}} |
| - | {{Lyon-INSA-ENS/ | + | {{Lyon-INSA-ENS/menuRealisationProtocolesFr}} |
<html> | <html> | ||
Revision as of 08:12, 22 August 2011
Fermentas digestion
This protocol aims at cutting plasmids on specific restriction sites using restriction enzymes.
Procedure
1. In an eppendorf tube, add the following solutions in any order :
-250 ng DNA ( if the concentration is unknown, use 5µL )
-2.5µL (1X) or 5µL (2X) 10X tango buffer ( this depends on the enzymes used )
-water to get a volume of 25mL after addition of the enzymes
2. Add 1µL of each one of the desired enzymes.
3. Incubate at 37°C for 1h30
4. Incubate at 70°C for 10 mn to inactivate the restriction enzymes.
