Team:Osaka/week3
From 2011.igem.org
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# Restriction digests of minipreppped parts | # Restriction digests of minipreppped parts | ||
# Gel electrophoresis of digests | # Gel electrophoresis of digests | ||
- | # Gel extraction of 1-1G,1-3A | + | # Gel extraction of 1-1G,1-3A |
# Transfer to LB culture medium:1-6I,07 | # Transfer to LB culture medium:1-6I,07 | ||
==August 20(Sat)== | ==August 20(Sat)== | ||
+ | # Gel electrophoresis of 1-5A | ||
+ | # Gel extraction of 1-5A | ||
+ | # Miniprep of yesterday's culture | ||
+ | # Restriction digests of minipreppped parts | ||
+ | # Gel electrophoresis of digests |
Revision as of 13:26, 20 August 2011
Contents |
August 16(Tue)
- Gel electrophoresis of 01,02,03,04,05,06
- Transfer to LB culture medium:01,02,03,04,05,06,1-23L
- Ligation
- 8/5 3. the same way
- Transformation of Ligation products
August 17(Wed)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Restriction digests of 03,05
- Gel electrophoresis of digests
- Ligation
- 07(C):05(upstream)+2-16N(downstream)+1-3A(vector)
- Transfer to LB culture midium:01,02,04,05,06
August 18(Thu)
- Transfer to LB culture midium:1-3A,1-5A,1-1G,03,1-23L
- Transformation of 07 and Resistry parts (See Table 3)
ID | Part Name | Resistance | Description |
---|---|---|---|
1-6I | <bbpart>BBa_R0040</bbpart> | A | TetR repressible promoter |
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
August 19(Fri)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Gel extraction of 1-1G,1-3A
- Transfer to LB culture medium:1-6I,07
August 20(Sat)
- Gel electrophoresis of 1-5A
- Gel extraction of 1-5A
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests