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Team:Caltech/Week 10
From 2011.igem.org
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===Results=== | ===Results=== | ||
The lacZ plated on LB-amp X-gal had about 520 colonies. The negative control had around 700 colonies. None of the colonies were blue, likely indicating that there was no functioning lacZ. Some were sent off for sequencing.<br/> | The lacZ plated on LB-amp X-gal had about 520 colonies. The negative control had around 700 colonies. None of the colonies were blue, likely indicating that there was no functioning lacZ. Some were sent off for sequencing.<br/> | ||
| + | ==August 18== | ||
| + | Restriction digest of R0010 and J23119 and their backbone for lacZ construct. Ligation of each promoter/backbone with lacZ purified yesterday. Transformed in XL-10 gold<br/> | ||
| + | ===Results=== | ||
| + | 4 colonies on pSB1C3 plate, all pink. <br/> | ||
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Revision as of 23:20, 18 August 2011
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Project |
August 15CPCR of Gibson pNT002 and pNT003 ResultsCPCR gels showed 200 bp bands, indicating self-ligation of all colonies picked. August 16Electrospray bisphenol A dissolved in methanol ResultsElectrospray results show correct mass spectrometry peaks
August 17Borrowed HindIII from Nate to be able to gel extract lacZ from the now smaller backbone pieces ResultsThe lacZ plated on LB-amp X-gal had about 520 colonies. The negative control had around 700 colonies. None of the colonies were blue, likely indicating that there was no functioning lacZ. Some were sent off for sequencing. August 18Restriction digest of R0010 and J23119 and their backbone for lacZ construct. Ligation of each promoter/backbone with lacZ purified yesterday. Transformed in XL-10 gold Results4 colonies on pSB1C3 plate, all pink.
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