Team:Osaka/week3

From 2011.igem.org

(Difference between revisions)
Line 27: Line 27:
!ID!!Part Name!!Resistance!!Description
!ID!!Part Name!!Resistance!!Description
|-
|-
-
|1-23L||<bbpart>BBa_R0040</bbpart>||A|| TetR repressible promoter
+
|1-6I||<bbpart>BBa_R0040</bbpart>||A|| TetR repressible promoter
|}
|}
# Miniprep of yesterday's culture
# Miniprep of yesterday's culture

Revision as of 13:33, 18 August 2011

Contents

August 16(Tue)

  1. Gel electrophoresis of 01,02,03,04,05,06
  2. Transfer to LB culture medium:01,02,03,04,05,06,1-23L
  3. Ligation
    • 8/5 3. the same way
  4. Transformation of Ligation products


August 17(Wed)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Restriction digests of 03,05
  5. Gel electrophoresis of digests
  6. Ligation
    • 07(C):05(upstream)+2-16N(downstream)+1-3A(vector)
  7. Transfer to LB culture midium:01,02,04,05,06


August 18(Thu)

  1. Transfer to LB culture midium:1-3A,1-5A,1-1G,03,1-23L
  2. Transformation of 07 and Resistry parts (See Table 3)
Table2
IDPart NameResistanceDescription
1-6I<bbpart>BBa_R0040</bbpart>A TetR repressible promoter
  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests

August 19(Fri)

August 20(Sat)

Retrieved from "http://2011.igem.org/Team:Osaka/week3"