Team:Freiburg/Notebook/18 August

From 2011.igem.org

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Take a picture of the gel, print picture and label the lanes!  
Take a picture of the gel, print picture and label the lanes!  
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<br/>
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===3A-assembly of Not-Gate, LovTAP in pSB1T3===
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<br/>
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===Investigators: Sophie===
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'''Digestion'''
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Amount of DNA and H20:
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{| cellpadding="10" cellspacing="0" border="1"
 +
|Sample
 +
|DNA μl
 +
|H20 μl
 +
|-
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|LovTAP
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|5
 +
|33
 +
|-
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|Not-Gate
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|2
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|36
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|-
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|pSB1T3
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|20
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|18
 +
|}
 +
 +
 +
Enzymes necessary for digestion:
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 +
{|cellpadding="10" cellspacing="0" border="1"
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|
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|LovTAP
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|Not-Gate
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|vector
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|-
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|enzyme 1
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|EcoRI
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|XbaI
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|EcoRI
 +
|-
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|enzyme 2
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|NheI
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|PstI
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|PstI
 +
|}
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 +
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*Incubation at 37°C for 6 hours
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*20 minutes at 80°C
{{:Team:Freiburg/Templates/footer}}
{{:Team:Freiburg/Templates/footer}}

Revision as of 13:08, 18 August 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

Miniprep

zymo Kit


Name:Sophie


Date:18.08.11
Continue from Experiment: Cloning: ε5 was empty and the products from the cloning of 12.08.11 contain GFP-pbd too. (Date): 12.08.11

(Name): Sophie

Project Name: inducible Promoter with pbd with cm-vector


Documentation:

Why are you doing this experiment? Name the parts which you extract.

ε5 was empty and the products from the cloning of 12.08.11 contain GFP-pbd too.

Name of the parts: GFP-pbd 4 4 4, GFP-pbd 4 1 3, GFP-pbd 6 6 8, GFP-pbd 4 2 2


Describe your results and mistakes and measure the DNA concentration with the Nanodrop and note the results.


GFP-pbd 4 4 4: 164,1 ng/µl

GFP-pbd 4 1 3: 100,8 ng/µl

GFP-pbd 6 6 8: 163,5 ng/µl

GFP-pbd 4 2 2: 144,4 ng/µl

How did you label your probes and where are they stored?


Labelled GFP-pbd 4 4 4, GFP-pbd 4 1 3, GFP-pbd 6 6 8, GFP-pbd 4 2 2

stored in -20 until sequencing


Testdigest

Name: Sophie


Date: 18.08.11
Continue from Experiment: Miniprep (Date): 18.08.11

(Name): Sophie

Project Name:inducible promoter for pbd with cm-vector

For one reaction you need: For Mastermix: Number of samples+2extra


4μl H2O 20
1μl Buffer, NEB4 5
1μl BSA (10x) 5
0,5 μl Enzym 1 2,5
0,5 μl Enzym 2 2,5
3 μl DNA

10 μl total volume


Give 3 μl of DNA in an eppi and add 7μl of the mastermix.

Incubate for about 1h at 37°C.


Add 1 μl Loading dye buffer and load the gel.

Take a picture of the gel, print picture and label the lanes!


3A-assembly of Not-Gate, LovTAP in pSB1T3


Investigators: Sophie

Digestion

Amount of DNA and H20:

Sample DNA μl H20 μl
LovTAP 5 33
Not-Gate 2 36
pSB1T3 20 18


Enzymes necessary for digestion:

LovTAP Not-Gate vector
enzyme 1 EcoRI XbaI EcoRI
enzyme 2 NheI PstI PstI


  • Incubation at 37°C for 6 hours
  • 20 minutes at 80°C

                       

Retrieved from "http://2011.igem.org/Team:Freiburg/Notebook/18_August"