Team:Freiburg/Notebook/17 August

From 2011.igem.org

(Difference between revisions)
(→green light receptor)
(→Commons)
Line 10: Line 10:
-
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Date: 25.07.11
+
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Date: 17.08.11
|-
|-
Line 54: Line 54:
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 1µl
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 1µl
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| DNA-Template
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| DNA-Template
-
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| PSB 1 A3
+
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"|  
-
 
+
-
PSB 1 C3
+
-
 
+
-
PSB 1 K3
+
PSB 1 T3
PSB 1 T3
Line 84: Line 80:
How did you label the PCR-Product, where is it stored and what do you do next?
How did you label the PCR-Product, where is it stored and what do you do next?
-
Labeled PSB 1 A3, PSB 1 C3, PSB 1 K3 and PSB 1 T3 stored in -20°C in last drawer
+
Labeled PSB 1 T3 stored in -20°C in last drawer
 +
 
==<span style="color:green;">green light receptor</span>==
==<span style="color:green;">green light receptor</span>==

Revision as of 13:34, 17 August 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

Commons

PCR


Name: Sophie


Date: 17.08.11
Continue from Experiment (Date)

(Name): Commons

Project Name: more linearized backbones (4 different vectors)

PCR-Mixture for one Reaction:

For a 50 µl reaction use


32,5µl H20 Name
10µl 5x Phusion Buffer of Primer
2.5µl Primer fw SB-prep-3P-1
2.5µl Primer dw SB-prep-2Ea
1µl dNTPs of Template DNA
1µl DNA-Template

PSB 1 T3

0.5 µl Phusion (add in the end)

What program do you use?

  1. 2Min 94°C
  2. 30s 94°C
  3. 30s 55°C
  4. 3min 72°C
  5. 10min 72°C

step 2,3 and 4 in 35 cycles


To confirm the PCR-Product has the correct size, load 2 µl of the sample onto an agarose-gel.


How did you label the PCR-Product, where is it stored and what do you do next?

Labeled PSB 1 T3 stored in -20°C in last drawer

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

blue light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME

                       

Retrieved from "http://2011.igem.org/Team:Freiburg/Notebook/17_August"