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Team:Lyon-INSA-ENS/Realisation/Week9
From 2011.igem.org
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M63 : NM522, S15, S3, S19, S4, S18 <br/><br/> | M63 : NM522, S15, S3, S19, S4, S18 <br/><br/> | ||
| - | Plating of individual or non individual clones from the previous NM522-pIG16 and NM522-pIG3 cultures | + | Plating of individual or non individual clones from the previous NM522-pIG16 and NM522-pIG3 cultures on LB+amp medium. Incubation at 37°C overnight.<br/><br/> |
| + | |||
| + | Preparation of 2X50mL LB/2 solutions. <br/> | ||
| + | |||
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<p style=" line-height : 1.5em"> | <p style=" line-height : 1.5em"> | ||
| + | Start of 3x5mL cultures of NM522 in LB medium from 50µL of a saturated NM522 culture for a later transformation.<br/> | ||
| + | Start of 5mL cultures of the individual clones plated on the previous day. <br/><br/> | ||
| + | |||
| + | Test of antibiotics : start of 5mL cultures ( 5mL LB, 50µL antibiotic, 10µL saturated NM522) of NM522 with antibiotics to test if the antibiotic is still good for Kan, Tet, Cm and Amp<br/> | ||
| + | The bacteria with Amp grow : a new solution is made ( 5x1mL mother solutions at 100mg/mL from solid Amp, and 2x10mL solutions at 100µg/mL by dilution of 10µL of mother solution into 10mL sterile water )<br/> | ||
| + | |||
</p> | </p> | ||
Revision as of 10:04, 17 August 2011
Week 9
From Monday the 15th of August to Friday the 19th of August 2011
Monday
Tuesday
Start of 5mL cultures for adherence tests from the collection :
LB : NM522
LB/2 : NM522, S3, S4, S15, S19
M63 : NM522, S15, S3, S19, S4, S18
Plating of individual or non individual clones from the previous NM522-pIG16 and NM522-pIG3 cultures on LB+amp medium. Incubation at 37°C overnight.
Preparation of 2X50mL LB/2 solutions.
Wednesday
Start of 3x5mL cultures of NM522 in LB medium from 50µL of a saturated NM522 culture for a later transformation.
Start of 5mL cultures of the individual clones plated on the previous day.
Test of antibiotics : start of 5mL cultures ( 5mL LB, 50µL antibiotic, 10µL saturated NM522) of NM522 with antibiotics to test if the antibiotic is still good for Kan, Tet, Cm and Amp
The bacteria with Amp grow : a new solution is made ( 5x1mL mother solutions at 100mg/mL from solid Amp, and 2x10mL solutions at 100µg/mL by dilution of 10µL of mother solution into 10mL sterile water )
Thursday
Friday
