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Team:UC Davis/Notebook/Week 10
From 2011.igem.org
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|'''Wild Type Controls''' || R0010+E0240+I13453+B0034+C0012 || R0051+E0240+I13453+B0034+C0051 || R0040E0240+I13453+B0034+C0040 | |'''Wild Type Controls''' || R0010+E0240+I13453+B0034+C0012 || R0051+E0240+I13453+B0034+C0051 || R0040E0240+I13453+B0034+C0040 | ||
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| + | In order to finish our construction, we are going to cut out R0010 and R0040 as inserts and put them in front of the parts needed. Tomorrow We will transform and hopefully have the finished construct by Wednesday. | ||
| + | |||
| + | --Tuesday 8/16/11-- | ||
| + | |||
| + | Ligated and transformed yesterday's digestion. | ||
Revision as of 21:47, 16 August 2011
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--Monday 8/15/11--
We got our sequences from last week in and checked them to make sure they looked good. Not all of the parts were correct, but we are about a day or two from being done with construction!
Here is a table of which parts are completed and which parts we need to complete in order to finish construction.
| LacI | c1 | TetR | |
| Repressor Screening | *R0010+E0240+I13453+B0034 | R0051+E0240+I13453+B0034 | *R0040+E0240+I13453+B0034 |
| Promoter Screening | E0240+I13453+B0034+C0012 | E0240+I13453+B0034+C0051 | E0240+I13453+B0034+C0040 |
| Wild Type Controls | *R0010+E0240+I13453+B0034+C0012 | R0051+E0240+I13453+B0034+C0051 | *R0040E0240+I13453+B0034+C0040 |
| ' | LacI | c1 | TetR |
| Repressor Screening | R0010+E0240+I13453+B0034 | R0051+E0240+I13453+B0034 | R0040+E0240+I13453+B0034 |
| Promoter Screening | E0240+I13453+B0034+C0012 | E0240+I13453+B0034+C0051 | E0240+I13453+B0034+C0040 |
| Wild Type Controls | R0010+E0240+I13453+B0034+C0012 | R0051+E0240+I13453+B0034+C0051 | R0040E0240+I13453+B0034+C0040 |
In order to finish our construction, we are going to cut out R0010 and R0040 as inserts and put them in front of the parts needed. Tomorrow We will transform and hopefully have the finished construct by Wednesday.
--Tuesday 8/16/11--
Ligated and transformed yesterday's digestion.
