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Team:Osaka/week3
From 2011.igem.org
(Difference between revisions)
(Created page with "{{Osaka}} ==August 16(Tue)== # Gel electrophoresis of 01,02,03,04,05,06 # Transfer to LB culture medium:01,02,03,04,05,06,1-23L # Ligation #* 8/5 3. the same way # Transformatio...") |
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#* 8/5 3. the same way | #* 8/5 3. the same way | ||
# Transformation of Ligation products | # Transformation of Ligation products | ||
| + | |||
| + | |||
| + | ==August 17(Wed)== | ||
| + | # Miniprep of yesterday's culture | ||
| + | # Restriction digests of minipreppped parts | ||
| + | # Gel electrophoresis of digests | ||
| + | # Restriction digests of 03,05 | ||
| + | # Gel electrophoresis of digests | ||
| + | # Ligation | ||
| + | #* 07(C):05(upstream)+2-16N(downstream)+1-3A(vector) | ||
| + | # Transfer to LB culture midium:01,02,03,04,05,06 | ||
Revision as of 13:19, 17 August 2011
August 16(Tue)
- Gel electrophoresis of 01,02,03,04,05,06
- Transfer to LB culture medium:01,02,03,04,05,06,1-23L
- Ligation
- 8/5 3. the same way
- Transformation of Ligation products
August 17(Wed)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Restriction digests of 03,05
- Gel electrophoresis of digests
- Ligation
- 07(C):05(upstream)+2-16N(downstream)+1-3A(vector)
- Transfer to LB culture midium:01,02,03,04,05,06
