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Team:Cornell/Week 11
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==Monday, August 15== | ==Monday, August 15== | ||
| - | Afternoon lab work done by: Youjin Cho | + | Afternoon lab work done by: Youjin Cho, Maneesh Gupta |
| - | :''' | + | :'''Objective''' |
| - | ::*Miniprep GFP samples that were cultured overnight and send them off | + | ::*Miniprep GFP samples that were cultured overnight and send them off for sequencing. |
::*Set-up the PCR for vioB using vio operon. | ::*Set-up the PCR for vioB using vio operon. | ||
:'''Miniprep & Sequencing''' | :'''Miniprep & Sequencing''' | ||
::*Miniprepped the GFP samples using standard Qiagen Miniprep protocol. | ::*Miniprepped the GFP samples using standard Qiagen Miniprep protocol. | ||
| - | :::GFP+ | + | :::GFP+Avi-Tag+pZE12 backbone (1)- 1,2,3 |
| - | :::GFP+ | + | :::GFP+Avi-Tag+pZE12 backbone (2)- 1,2,3 |
::*Set-up the samples for sequencing using the reverse primer. | ::*Set-up the samples for sequencing using the reverse primer. | ||
:::Order number: 10254977 | :::Order number: 10254977 | ||
| - | :'''PCR | + | :'''PCR Reaction''' |
::*Set-up the vioB PCR using the vio operon (=20.3ng/μL). | ::*Set-up the vioB PCR using the vio operon (=20.3ng/μL). | ||
::*As Didi suggested, used the melting temperature of 53°C and annealing time for 3:30 minutes. | ::*As Didi suggested, used the melting temperature of 53°C and annealing time for 3:30 minutes. | ||
Revision as of 20:11, 15 August 2011
Week 1 | Week 2 - 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |
August 14th - August 20th
Sunday, August 14
Afternoon lab work done by: Charlie Chung
- Picked three colonies from the two (GFP + Avi-Tagged pZE12 backbone) plates
- - GFP + Avi + BB (1) #1, 2, 3
- - GFP + Avi + BB (2) #1, 2, 3
- Incubating overnight in 37°C shaker of Room 304
- Re-picked three colonies from the (RFP + Avi-Tagged pZE12 backbone) plate in order to retry subculturing, induction, and cell lysis
- - RFP + Avi + BB #7, 8, 9
- Incubating overnight in 37°C shaker of Room 304
Monday, August 15
Afternoon lab work done by: Youjin Cho, Maneesh Gupta
- Objective
- Miniprep GFP samples that were cultured overnight and send them off for sequencing.
- Set-up the PCR for vioB using vio operon.
- Miniprep & Sequencing
- Miniprepped the GFP samples using standard Qiagen Miniprep protocol.
- GFP+Avi-Tag+pZE12 backbone (1)- 1,2,3
- GFP+Avi-Tag+pZE12 backbone (2)- 1,2,3
- Set-up the samples for sequencing using the reverse primer.
- Order number: 10254977
- PCR Reaction
- Set-up the vioB PCR using the vio operon (=20.3ng/μL).
- As Didi suggested, used the melting temperature of 53°C and annealing time for 3:30 minutes.
