Team:Wisconsin-Madison/notebookcalender

From 2011.igem.org

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The primary source of results thus far has been a plate reader. This is a device which can record the optical density (OD – a measure of cell concentration) as well as the fluorescence of up to 96 cultures at once. Ideally, we hope to produce such a robust fluorescent response as to not need the plate reader for qualitative assessments.
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This graph shows the fluorescent response of cell cultures containing two plasmids: pBAD33bb: exaDE and PexaA: TagRFP in pSB1A2 (pEtRv2.1). The fluorescence of the system at varying ethanol concentrations was normalized to the optical density of their respective cell culture. The cells’ fluorescence was measured over a range of ethanol concentrations from 0%-5% EtOH. This ethanol sensing system is “turned on” or induced in the presence of arabinose by the PBAD promoter. The blue data points on the graph represent the fluorescence of cell cultures without any arabinose and therefore the ethanol sensor is uninduced; the red data points represent the fluorescent response of the induced system in the presence of .2% arabinose.  
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<a name="Schedule"></a><h2> <span class="mw-headline">Schedule</span></h2>
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<img src="https://static.igem.org/mediawiki/2011/0/00/Pets_2.1_graph.jpg" align="left" width="500">
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From the graph, it can be seen that there is an increase in fluorescence between the induced and uninduced system. This is the result that was expected, however, there isn’t a large difference in fluorescence between the two systems. To further increase this difference in fluorescence, we intend to decrease the “leakiness” of our promoter through directed evolution.  Additionally, the graph shows a positive correlation between fluorescence and ethanol concentration; therefore, our ethanol sensor exhibits a linear response in fluorescent intensity with varying ethanol concentrations. The magnitude of the slope of the data points represents the degree of our systems response. Currently, the slope of the data is smaller than desired. This will  also be the target of a directed evolution upon the ethanol sensor.
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<ul><li> Week of May 10th – all DNA requests (strains, plasmids, primers, synthesis) must be submitted
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</li><li> Week of July 15th – presentation outline and first draft of background section will be presented at the weekly meeting
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</li><li> Week of August 15th – First draft of research presentation will be given in weekly meeting
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</li><li> August 16 - Project Description
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</li><li> August 27th – T-shirt design should be finalized so we can submit the order.
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</li><li> September 15th – Second draft of research presentation will be given in weekly meeting, poster outline should be completed.
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</li><li> September 20th - Project Abstract, Track, Team Roster, attendance fees due to HQ
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</li><li> October 27th - <b>Judging Form</b>, <b>PROJECT &amp; PARTS SUBMISSION</b>, <b>DNA SUMBMISSION</b>
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</li><li> October 27th, 11:59pm EST - <b>Wiki Freeze</b>
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</li><li> October 15th – Final Poster is submitted for printing.
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</li><li> November 5th – 8th - UW iGEM takes the Jamboree by storm!
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</li></ul>
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</p>
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<a name="Notebooks"></a><h2> <span class="mw-headline">Notebooks</span></h2>
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<ul><li> <a href="/Team:Wisconsin-Madison/notebook/Sarah" title="Team:Wisconsin-Madison/notebook/Sarah">Sarah's Notebook</a>
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</li><li> <a href="/Team:Wisconsin-Madison/notebook/Nate" title="Team:Wisconsin-Madison/notebook/Nate">Nate's Notebook</a>
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</li><li> <a href="/Team:Wisconsin-Madison/notebook/Yue" title="Team:Wisconsin-Madison/notebook/Yue">Yue's Notebook</a>
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</li><li> <a href="/Team:Wisconsin-Madison/notebook/Peter" title="Team:Wisconsin-Madison/notebook/Peter">Peter's Notebook</a>
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</li><li> <a href="/Team:Wisconsin-Madison/notebook/Mary" title="Team:Wisconsin-Madison/notebook/Mary">Mary's Notebook</a>
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</li><li> <a href="/Team:Wisconsin-Madison/notebook/Justin" title="Team:Wisconsin-Madison/notebook/Justin">Justin's Notebook</a>
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</li></ul>
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Revision as of 14:34, 15 August 2011









Notebook >> Calender, Protocols, References

Ethanol Sensor

Schedule

  • Week of May 10th – all DNA requests (strains, plasmids, primers, synthesis) must be submitted
  • Week of July 15th – presentation outline and first draft of background section will be presented at the weekly meeting
  • Week of August 15th – First draft of research presentation will be given in weekly meeting
  • August 16 - Project Description
  • August 27th – T-shirt design should be finalized so we can submit the order.
  • September 15th – Second draft of research presentation will be given in weekly meeting, poster outline should be completed.
  • September 20th - Project Abstract, Track, Team Roster, attendance fees due to HQ
  • October 27th - Judging Form, PROJECT & PARTS SUBMISSION, DNA SUMBMISSION
  • October 27th, 11:59pm EST - Wiki Freeze
  • October 15th – Final Poster is submitted for printing.
  • November 5th – 8th - UW iGEM takes the Jamboree by storm!


Notebooks



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