Solution II
From 2011.igem.org
(Difference between revisions)
m (correct % with substitution of N) |
(replace % with N) |
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Line 5: | Line 5: | ||
The ideal Solution II would be freshly made, using 10 N NaOH. | The ideal Solution II would be freshly made, using 10 N NaOH. | ||
- | To make 5 ml of Solution II using 0.4 | + | To make 5 ml of Solution II using 0.4 N NaOH stock and 10% SDS stock solution we use: |
2.50 ml 0.4 N NaOH | 2.50 ml 0.4 N NaOH | ||
0.05 ml 10% SDS | 0.05 ml 10% SDS | ||
2.45 ml distilled water or NF water | 2.45 ml distilled water or NF water |
Revision as of 02:36, 15 August 2011
0.2 N NaOH [Think M when you see N] 1.0% SDS
The ideal Solution II would be freshly made, using 10 N NaOH.
To make 5 ml of Solution II using 0.4 N NaOH stock and 10% SDS stock solution we use:
2.50 ml 0.4 N NaOH 0.05 ml 10% SDS 2.45 ml distilled water or NF water