Team:Freiburg/Notebook/13 August

From 2011.igem.org

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===NAME OF YOUR EXPERIMENT===
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===Miniprep===
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'''Investigators: NAME'''
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'''Investigators: Rüdiger'''
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Miniprep of pGex-GP-1.
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Revision as of 13:11, 13 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

Ligation

Investigators: Sandra

Ligation of LovTAP, Not-Gate in pSB1T3.

Stored in the freezer in Gibson stuff.
Next step: Transformation

red light receptor

MIniprep

Investigators: Sandra

Miniprep of:

  • 1pTd 1/2 (PR1+pcyA+terminator)
  • 2pTd 1/2(PR2+pcyA+terminator)
  • 1pTb 1/2(PR1+pcyA+terminator)
  • 2pTb 1/2(PR2+pcyA+terminator)
  • 1L23 1/2(PR1+ho1+terminator)
  • 2L23 1/2(PR2+ho1+terminator)

and S54a,b,c,d.


Sample DNA concentration
M54a 181.0
M54b 224.9
M54c 222.0
M54d 205.9
1pTb1 27.2
1pTb2 17.9
2pTb1 33.2
2pTb2 27.6
1pTd1 40.9
1pTd2 33.6
2pTd1 37.9
2pTd2 37.7
1L231 15.3
1L232 37.3
2L231 25.2
2L232 24.1

stored in the undigested miniprep box III.

Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

Miniprep

Investigators: Rüdiger

Miniprep of pGex-GP-1.

                       

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