Team:Freiburg/Notebook/12 August

From 2011.igem.org

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'''Investigators: Sandra'''
'''Investigators: Sandra'''
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To confrim the PCR worked this time, we loaded the sample onto a gel. The size of the fragment should be 3000bp.
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To confrim the PCR worked this time, we loaded the sample onto a gel. The size of the fragment should be 1000bp.
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The gel showed a band at 1000bp.
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We digested the PCR product with DpnI and then purified the DNA with the PCR purification kit.
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===3A-assembly of Not-Gate, LovTAP in pSBiT3===
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'''Investigators: Sandra'''
==<span style="color:red;">red light receptor</span>==
==<span style="color:red;">red light receptor</span>==

Revision as of 15:04, 12 August 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

Meeting

attendants:
Time:

green light receptor

already done:


To-do:

blue light receptor

already done:


To-do:

red light receptor

already done:


To-do:

Lysis cassette

already done:


To-do:


Precipitator

already done:


To-do:

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

Gel of the PCR product: LovTAP

Investigators: Sandra

To confrim the PCR worked this time, we loaded the sample onto a gel. The size of the fragment should be 1000bp. The gel showed a band at 1000bp. We digested the PCR product with DpnI and then purified the DNA with the PCR purification kit.


3A-assembly of Not-Gate, LovTAP in pSBiT3

Investigators: Sandra

red light receptor

Picking clones of pcyA and ho1

Investigators: Sandra

Picked 2 clones of each plate:

  • 1pTd (PR1+pcyA+terminator)
  • 2pTd (PR2+pcyA+terminator)
  • 1pTb (PR1+pcyA+terminator)
  • 2pTb (PR2+pcyA+terminator)
  • 1L23 (PR1+ho1+terminator)
  • 2L23 (PR2+ho1+terminator)

Incubation over night.

Lysis cassette

Miniprep

Investigators: Jakob, Ruediger, Theo


Name: Jakob, Ruediger, Theo Date: 12.08.2011
Continue from Experiment: Transformation, 10.08.2011
Project Name:

Lysis systeme, plasticbinding, GST-Tag, IPTG inducible promotor, LOV-Tap

Documentation:

Why are you doing this experiment? Name the parts which you extract.

  • Need the DNA concentration

Describe your results and mistakes and measure the DNA concentration with the Nanodrop and note the results. How did you label your probes and where are they stored?


Sample ID Nuclein Acid Conc. ng/µl
A260/280
A260/230
LOV 3A 81,8 1,88 2,23
PR4+GFP 87,1 1,85 1,93
PR6-1 124,7 1,89 2,19
PR6-2 138,1 1,88 2,14
PR6-3 67,2 1,94 2,09
PR6-4 96,5 1,85 1,58
PR6-5 102,7 1,94 2,14
PR6-6 111,5 1,88 1,99
PR6-7 40,1 1,99 1,94
PR4-1 71,3 1,90 1,79
PR4-2 119,6 1,83 1,69
PR4-3 135,4 1,84 1,83
PR4-4 99,2 1,86 1,85
PR4-5 90,9 1,84 1,67
Lys+RBS-1 33,1 1,99 1,87
Lys+RBS-2 43,8 1,94 1,91
Lys+RBS-3 27,7 1,98 1,82
Lys+RBS-4 38,9 2,02 1,92
Lys+RBS-5 31,0 2,03 1,92
Lys+RBS-6 39,9 2,00 1,84
PGEX6P1-a 65,9 1,94 2,02
PGEX6P1-b 65,3 1,90 1,98
PGEX6P1-c 70,6 1,95 2,05
PGEX6P1-d 43,6 1,82 1,40
PGEX6P1-e 53,9 1,87 1,75
S54 219,6 1,87 2,21
S54 216,4 1,88 2,21

Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME

                       

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