Team:SouthBend-Mishawaka-HS/Notebook

From 2011.igem.org

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=May=
=May=
==May18==
==May18==
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Poured 12 LB AMP plates:(2mg of Ampicillin to 250ml of Agar)<br>
+
(1)Poured 12 LB AMP plates:(2mg of Ampicillin to 250ml of Agar)<br>
-
Obtained a vial of 2 microliters of TOP-10 electrocompetant <i>E. coli</i> cells (44-0003/793762)<br>
+
(2)Obtained a vial of 2 microliters of TOP-10 electrocompetant <i>E. coli</i> cells (44-0003/793762)<br>
-
Added 50 microliters of pure water from the Gemini machine<br>
+
(3)Added 50 microliters of pure water from the Gemini machine<br>
-
Added 10 ul of water to well 6E of plate 2, which contained part F2620. Put 2 ul of the diluted DNA and placed in 50 ul of transformation solution (50mm CaCl2, pH 6.1).<br>
+
(4)Added 10 ul of water to well 6E of plate 2, which contained part F2620.<br>
-
Incubate on ice for 5 minutes<br>
+
(5)Added 2 ul of the diluted DNA in 50 ul of transformation solution (50mm CaCl2, pH 6.1 BIO-RAD Transformation Solution control # 31000008916 recieved 1-17-11 GT).<br>
-
Heat shocked at 40 degrees Celcius for 50 seconds<br>
+
(6)Incubate on ice for 5 minutes<br>
-
Incubated at 4 degrees Celcius for 5 minutes<br>
+
(7)Heat shocked at 40 degrees Celcius for 50 seconds<br>
-
Added 1mL of LB (5-17-11 DG)<br>
+
(8)Incubated at 4 degrees Celcius for 5 minutes<br>
 +
(9)Added 1mL of LB (5-17-11 DG)<br>
   
   
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Revision as of 21:42, 19 May 2011

May

May18

(1)Poured 12 LB AMP plates:(2mg of Ampicillin to 250ml of Agar)
(2)Obtained a vial of 2 microliters of TOP-10 electrocompetant E. coli cells (44-0003/793762)
(3)Added 50 microliters of pure water from the Gemini machine
(4)Added 10 ul of water to well 6E of plate 2, which contained part F2620.
(5)Added 2 ul of the diluted DNA in 50 ul of transformation solution (50mm CaCl2, pH 6.1 BIO-RAD Transformation Solution control # 31000008916 recieved 1-17-11 GT).
(6)Incubate on ice for 5 minutes
(7)Heat shocked at 40 degrees Celcius for 50 seconds
(8)Incubated at 4 degrees Celcius for 5 minutes
(9)Added 1mL of LB (5-17-11 DG)

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