Team:Freiburg/Notebook/9 August

From 2011.igem.org

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==<span style="color:green;">green light receptor</span>==
==<span style="color:green;">green light receptor</span>==
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===NAME OF YOUR EXPERIMENT===
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===Results Transformation===
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'''Investigators:NAME'''
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We dont see any colonies
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*To-do: do quickchange again.
==<span style="color:blue;">blue light receptor</span>==
==<span style="color:blue;">blue light receptor</span>==

Revision as of 10:56, 17 August 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

Results Transformation

We dont see any colonies

  • To-do: do quickchange again.

blue light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

NAME OF YOUR EXPERIMENT

new 3A assenbly with Amp Vector

Investigators: Sophie

3A assembly

The last cloning of Theo and Jakob produced one clone with GFP-plastic-binding-domain (GFP-pbd) inside but without Promoter-Ribosome-binding site (PR). So I tried again to combine the GFP-pbd with PRs via 3a assenbly. I used two different PRs (PR4 and PR6) with different RBS binding strenght. Name of the samples: ε5 (GFP-pbd), pSB1A3, PR4, PR6. stored in "Minipreps, verdaut"-box

Ligation

I ligated ε5 (GFP-pbd) to PR4 or PR6 and to the Amp-Vector. Therefore I once took the ratio 3:1 insert to vector and once the ration 1:1 insert to vector.

                       

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