Team:Freiburg/Notebook/9 August

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(Precipitator)
(NAME OF YOUR EXPERIMENT)
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The last cloning of Theo and Jakob produced one clone with GFP-plastic-binding-domain (GFP-pbd) inside but without Promoter-Ribosome-binding site (PR). So I tried again to combine the GFP-pbd with PRs via 3a assenbly. I used two different PRs (4 and 6) with different RBS binding strenght.
The last cloning of Theo and Jakob produced one clone with GFP-plastic-binding-domain (GFP-pbd) inside but without Promoter-Ribosome-binding site (PR). So I tried again to combine the GFP-pbd with PRs via 3a assenbly. I used two different PRs (4 and 6) with different RBS binding strenght.
Name of the samples:
Name of the samples:
-
ε5
+
ε5,
-
pSB1A3
+
pSB1A3,
-
PR4
+
PR4,
-
PR6
+
PR6.
stored in "Minipreps, verdaut"-box
stored in "Minipreps, verdaut"-box
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Revision as of 10:15, 10 August 2011


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