Team:KAIST-Korea

From 2011.igem.org

(Difference between revisions)
Line 70: Line 70:
<BODY onLoad="getTime()" oncontextmenu="return false" ondragstart="return false" onselectstart="return false">
<BODY onLoad="getTime()" oncontextmenu="return false" ondragstart="return false" onselectstart="return false">
-
 
-
<script language="javascript">
 
-
window.onload = function() {
 
-
document.onselectstart = function() {return false;} // for ie
 
-
document.onmousedown = function() {return false;} // for mozilla
 
-
}
 
-
</script>
 
<div id="contentin">
<div id="contentin">

Revision as of 08:45, 30 August 2011

Menu Menu

Project Description


E. Casso is an E. coli system engineered into art. This system consists of two types of genetically modified E. coli: the random signal generating E. coli (the “Brush E. coli”) and the color generating E. coli (the “Paint E. coli”).

The Brush E. coli possesses genes (lasI, luxI, rhlI, and cinI) that produce quorum in its chromosomal DNA. Each Brush E. coli will secrete quorum molecules randomly associated with one of four colors among red, cyan, yellow, and green by a modified Cre-loxP mechanism.

The Paint E.coli possesses plasmid vectors that express one of four colors of fluorescent proteins in response to the type of quorum it receives from the Brush E.coli. The quorum sensing complex completed in the Brush will bind to the ribosome binding site (RBS) of the corresponding fluorescent gene.

Using this system, we can create art on an LP plate with the cells’ “random” contribution.

>> go to Safety Issues

Retrieved from "http://2011.igem.org/Team:KAIST-Korea"