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Sensor: Week 11 July 25-29
From 2011.igem.org
(Difference between revisions)
(Created page with "==Monday, July 25== {|border="1" !Protocol |align="center" colspan="2"|'''Part Involved with Protocol''' |- |rowspan="2"|Restriction Digest |Insert using EcoRI and SpeI: |B0034 |...") |
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|Transformation/Plating | |Transformation/Plating | ||
|colspan="2"|The above ligation was transformed into<br />Escherichia coli cells and plated onto<br />kanamycin resistant plates. | |colspan="2"|The above ligation was transformed into<br />Escherichia coli cells and plated onto<br />kanamycin resistant plates. | ||
| + | |} | ||
| + | O<sub>R</sub>+K64800 and O<sub>R</sub> were both grown up in culture to be used as freezer stocks. | ||
| - | + | ==Tuesday, July 26== | |
| + | {|border="2" | ||
| + | !Protocol | ||
| + | |align="center" colspan="2"|'''Part Involved with Protocol''' | ||
| + | |- | ||
| + | |rowspan="2"|Restriction Digest | ||
| + | |Inserts using EcoRI and SpeI: | ||
| + | |B0031 + C0051 (R)<br />B0030 + C0051 (R)<br />B0034 + C0051 (R) | ||
| + | |- | ||
| + | |Vector using EcoRI and XbaI: | ||
| + | |O<sub>R</sub>+K648000 | ||
| + | |- | ||
| + | |Ligation | ||
| + | |colspan="2"|B0030 + C0051 + Or + K648000<br />B0031 + C0051 + Or + K648000<br />B0034 + C0051 + Or + K648000 | ||
| + | |- | ||
| + | |Transformation/Plating | ||
| + | |colspan="2"|The above ligations weretransformed into<br />Escherichia coli cells and plated onto<br />kanamycin resistant plates. | ||
| + | |} | ||
[[Team:Penn_State/Notebook| Back to Notebook]] | [[Team:Penn_State/Notebook| Back to Notebook]] | ||
Revision as of 19:32, 4 August 2011
Monday, July 25
| Protocol | Part Involved with Protocol | |
|---|---|---|
| Restriction Digest | Insert using EcoRI and SpeI: | B0034 |
| Vector using EcoRI and XbaI: | tev protease | |
| Ligation | B0034+tev protease | |
| Transformation/Plating | The above ligation was transformed into Escherichia coli cells and plated onto kanamycin resistant plates. | |
OR+K64800 and OR were both grown up in culture to be used as freezer stocks.
Tuesday, July 26
| Protocol | Part Involved with Protocol | |
|---|---|---|
| Restriction Digest | Inserts using EcoRI and SpeI: | B0031 + C0051 (R) B0030 + C0051 (R) B0034 + C0051 (R) |
| Vector using EcoRI and XbaI: | OR+K648000 | |
| Ligation | B0030 + C0051 + Or + K648000 B0031 + C0051 + Or + K648000 B0034 + C0051 + Or + K648000 | |
| Transformation/Plating | The above ligations weretransformed into Escherichia coli cells and plated onto kanamycin resistant plates. | |
