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Team:Paris Liliane Bettencourt/Notebook/2011/07/18/
From 2011.igem.org
(Difference between revisions)
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<p style="display:block;"><u>Restriction Digest of RBS-spoVg and T7 RNA polymerase </u> | <p style="display:block;"><u>Restriction Digest of RBS-spoVg and T7 RNA polymerase </u> | ||
<ul><li>BBa_K145001 cut by XbaI and PstI | <ul><li>BBa_K145001 cut by XbaI and PstI | ||
| - | </li><li> | + | </li><li>BBa_K143021 cut by SpeI and PstI |
<br />Final volume : 20 µL | <br />Final volume : 20 µL | ||
| + | |||
| + | </p><p style="display:block;"><u>DNA Gel electrophoresis (T7 RNA polymerase)</u> | ||
| + | </p><p style="display:block;"><font color="blue">Protocol</font> (6 wells) | ||
| + | </p> | ||
| + | <ul><li>1kb ladder | ||
| + | </li><li>T7 undigested plasmid | ||
| + | </li><li>Digested T7 (BBa_K145001 cut by XbaI and PstI) | ||
| + | |||
| + | </li></ul> | ||
| + | |||
| + | [[File:T7 electro 20072011]] | ||
Revision as of 13:48, 4 August 2011
Edward
Minipreps
- on BBa_I712074 (pT7), concentration of DNA=249.5ng/µL
- on BBa_K145001 (T7 RNA polymerase), concentration of DNA=220.4ng/µL
- on BBa_K143021 (RBS-spoVg colony 17), concentration of DNA=165.9ng/µL
Restriction Digest of RBS-spoVg and T7 RNA polymerase
- BBa_K145001 cut by XbaI and PstI
- BBa_K143021 cut by SpeI and PstI
Final volume : 20 µL</p><p style="display:block;">DNA Gel electrophoresis (T7 RNA polymerase) </p><p style="display:block;">Protocol (6 wells) </p>
- 1kb ladder
- T7 undigested plasmid
- Digested T7 (BBa_K145001 cut by XbaI and PstI)
