Team:Freiburg/Notebook/5 July

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==<span style="color:green;">green light receptor</span>==
==<span style="color:green;">green light receptor</span>==
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==Purification==
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'''Investigators: Julia'''
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Purification of digested PCR products with the PCR purification-Kit.
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<br/>
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<br/>
===Ligation of Ccas in pSB1C3===
===Ligation of Ccas in pSB1C3===

Revision as of 11:07, 2 August 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

Purification

Investigators: Julia

Purification of digested PCR products with the PCR purification-Kit.

Ligation of Ccas in pSB1C3

Investigators: Julia

Ligated Ccas and pSB1C3 and then transformed the cells with the ligated vector.

Transformation of PCR product Ccas

Investigators: Jakob

Comments: Colonies had to be picked.

blue light receptor

Transformation of Lov-Tap

Investigators: Jakob

  • Lov-Tap1 (blue light sensor (BBa_K360121) in pSB1C3)
  • Lov-Tap2 (Trp promotor with mcherry reporter gene in pSB1C3)
  • Lov-Tap3 (Lov-Tap plus Trp promotor with mcherry reporter gene in pSB1C3)



Precipitator

Gel of digested parts and ligation

Investigators: Rügiger

Gel of digested parts:

  • Vector: pSB1K3
  • GFP-plastic-binding-domain
  • Promotor-RBS (PR1-PR6)


Comments: GFP was excised. Other inserts were not stained properly.

Ligation of PR1 and GFP-plastic-binding-domain in pSB1K3.

                       

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