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Team:Caltech/Week 8
From 2011.igem.org
(Difference between revisions)
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==July 31== | ==July 31== | ||
Started a LB+amp overnight culture of the B0034 glycerol stock from the 2010 Caltech iGEM Team<br/> | Started a LB+amp overnight culture of the B0034 glycerol stock from the 2010 Caltech iGEM Team<br/> | ||
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==August 1== | ==August 1== | ||
PCR pSB3K3 and pNT003 insert with the new primers.<br/> | PCR pSB3K3 and pNT003 insert with the new primers.<br/> | ||
Gel extract the pNT002 insert from the July 29 PCR<br/> | Gel extract the pNT002 insert from the July 29 PCR<br/> | ||
Minimal media transfers<br/> | Minimal media transfers<br/> | ||
| - | Transformation of pUC19 into competent cells for cell dry weight experiment<br/> | + | Transformation and plating of pUC19 into competent cells for cell dry weight experiment<br/> |
| + | Overnight culture of B0034 glycerol stock<br/> | ||
===Results=== | ===Results=== | ||
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<td>12.5</td> | <td>12.5</td> | ||
</tr> | </tr> | ||
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</table> | </table> | ||
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==August 2== | ==August 2== | ||
Gibson assemble pNT002 using the gel extracted insert and linearized pSB3K3<br/> | Gibson assemble pNT002 using the gel extracted insert and linearized pSB3K3<br/> | ||
Gibson assemble pNT003 using the gel extracted insert and linearized pSB4A5<br/> | Gibson assemble pNT003 using the gel extracted insert and linearized pSB4A5<br/> | ||
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}} | }} | ||
Revision as of 01:04, 2 August 2011
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Project |
July 31Started a LB+amp overnight culture of the B0034 glycerol stock from the 2010 Caltech iGEM Team August 1PCR pSB3K3 and pNT003 insert with the new primers. ResultsThe enrichment culture plates show no visible growth yet.
August 2Gibson assemble pNT002 using the gel extracted insert and linearized pSB3K3
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