Team:Michigan/Protocols

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Latest revision as of 22:31, 28 September 2011

Protocols

Basic protocols ::: These protocols are necessary for all lab operations.

Autoclave usage

OD600 optimization


Gels and electrophoresis ::: These protocols contain information on the production and usage of gels.

Gel electrophoresis

Using the gel viewer


Transformation ::: These protocols contain information on the production, usage, and transformation of competent cells.

Production of electrocompetent cells

Electroporation of electrocompetent cells

Production of standard/QT competent cells

Quick Transformation (QT) of standard competent cells

Heat shock transformation of standard competent cells.


Enzyme usage ::: These protocols contain information on digestion, ligation, and specific information on enzymatic operations.

Using quick ligase

Using T4 ligase

Using Restriction enzymes


Biobrick specific ::: These protocols contain information specific to the usage of Biobricks.

Extraction of BioBricks from wells in the distribution