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Team:Caltech/Week 8
From 2011.igem.org
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Gel extract the pNT002 insert from the July 29 PCR<br/> | Gel extract the pNT002 insert from the July 29 PCR<br/> | ||
Gibson assemble the PCR'd pNT003 insert and pSB4A5 together if the insert is at a sufficient concentration<br/> | Gibson assemble the PCR'd pNT003 insert and pSB4A5 together if the insert is at a sufficient concentration<br/> | ||
| + | Minimal media transfers<br/> | ||
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| + | ===Results=== | ||
| + | The enrichment culture plates show no visible growth yet. | ||
| + | |||
==August 2== | ==August 2== | ||
Gibson assemble pNT002 using the gel extracted insert and linearized pSB3K3<br/> | Gibson assemble pNT002 using the gel extracted insert and linearized pSB3K3<br/> | ||
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Revision as of 21:43, 1 August 2011
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Project |
July 31Started a LB+amp overnight culture of the B0034 glycerol stock from the 2010 Caltech iGEM Team August 1PCR pSB3K3 and pNT003 insert with the new primers. ResultsThe enrichment culture plates show no visible growth yet. August 2Gibson assemble pNT002 using the gel extracted insert and linearized pSB3K3
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