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Revision as of 21:18, 28 July 2011 (view source) Mduley (Talk | contribs) ← Older edit		Revision as of 02:35, 15 August 2011 (view source) DrLiz (Talk | contribs) (Making Solution II for Plasmid Protein Purification) Newer edit →
Line 1:		Line 1:
-	0.2 M NaOH	+
-	1%   SDS	+	0.2 N NaOH   [Think M when you see N]
		+	1.0% SDS
		+
		+	The ideal Solution II would be freshly made, using 10 N NaOH.
-	2.5 ml of 0.4 M NaOH	+	To make 5 ml of Solution II using 0.4% NaOH stock and 10% SDS stock solution we use:
-	0.05 ml of 10% SDS	+	2.50 ml 0.4% NaOH
-	2.45 ml Nuclease free Water	+	0.05 ml 10% SDS
-		+	2.45 ml distilled water or NF water
-	lends 5 mls of solution	+

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Revision as of 02:35, 15 August 2011

0.2 N NaOH    [Think M when you see N]
1.0% SDS

The ideal Solution II would be freshly made, using 10 N NaOH.

To make 5 ml of Solution II using 0.4% NaOH stock and 10% SDS stock solution we use:

 2.50 ml 0.4% NaOH
 0.05 ml 10% SDS
 2.45 ml distilled water or NF water