Team:IIT Madras

From 2011.igem.org

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Google Insights suggests the most number of searches for “synthetic biology” is from India.<br/><br/>
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Yo! Veni, Vidi, Veci!<br/><br/>
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<h1>And then E.coli said, <i>"Let there be light!"</i></h1>
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So what do we have in store for iGEM this year?<br/>
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<b>Proteorhodopsin, The Savior</b>
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Proteorhodopsin belongs to the class of Bacterial rhodopsin, membrane proteins which are photon driven proton pumps. And its potential application ranges from increased metabolic activity to usage as a solar cell. After weeks of literature survey and brainstorming we came up with the following novel applications of Proteorhodopsin in Synthetic Biology.
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Most research and development of concepts to date has dealt with gene networks. These networks are said to be synthetic because they are novel and designed by humans, never seen in nature. This is analogous to building electronic circuits from individual components. <br/>Recent research has however shown success in developing novel proteins and enzymes which have been designed based on the modularity of proteins. We plan to use the same principle to design an expression system that can be modified on the protein level so that it can be activated by a molecule of our choice. We want to design a novel signal transduction pathway, where the surface receptor can be modified by simply replacing the extracellular sensory domain alone, with little no change in the output pattern. This could be analogous to development of novel electronic components with novel functions. <br/><br/>
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1.Light based Screening for Transformants (Light Saber)
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<br/>2.Enhancing Recombinant Protein synthesis (Super active Bacteria)
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<br/>3.Improving Hyularonic Acid Production (E.plasti)
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<br/>4.Increase in Biofuel production (Butanol biosynthesis)
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<br/>5.Light-switch life form (Photographic film)
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<br/>In other words, we are giving bacteria the ability to utilize the cheapest, cleanest and the most abundant substrate - a photon, to accomplish what a normal bacteria can't. The possibilities are limitless.
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[[Image:IITM_Two_Complement_System.png|500px|center|]]
 
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We plan to target this problem by accessing the modularity found in the proteins involved in the two-component signalling pathways of prokaryotes. There are over 250 different pathways that have already been studied to a very large extent and thousands of proteins have been characterized wrt their domain architecture and their functionality.<br/>
 
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Of the many signalling pathways that have been studied, the EnvZ/OmpR Two component system of ''E.Coli'' is one of the first few to be understood. It is found to be representative of the basic domain architecture found in many of the Two Component systems. <br/>
 
Watch this space for more updates…
Watch this space for more updates…

Revision as of 21:02, 16 August 2011

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And then E.coli said, "Let there be light!"


Proteorhodopsin, The Savior

Proteorhodopsin belongs to the class of Bacterial rhodopsin, membrane proteins which are photon driven proton pumps. And its potential application ranges from increased metabolic activity to usage as a solar cell. After weeks of literature survey and brainstorming we came up with the following novel applications of Proteorhodopsin in Synthetic Biology.

1.Light based Screening for Transformants (Light Saber)
2.Enhancing Recombinant Protein synthesis (Super active Bacteria)
3.Improving Hyularonic Acid Production (E.plasti)
4.Increase in Biofuel production (Butanol biosynthesis)
5.Light-switch life form (Photographic film)

In other words, we are giving bacteria the ability to utilize the cheapest, cleanest and the most abundant substrate - a photon, to accomplish what a normal bacteria can't. The possibilities are limitless.


Watch this space for more updates…

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