Team:Caltech/Week 7
From 2011.igem.org
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File:DDT_noV_1.jpg|DDT no V | File:DDT_noV_1.jpg|DDT no V | ||
File:DDT_noV_2.jpg|DDT no V | File:DDT_noV_2.jpg|DDT no V | ||
- | File: | + | File:BPA_+V_roomT_1.jpg|BPA room temperature +V location 1 |
- | File: | + | File:BPA_+V_roomT_2.jpg|BPA room temperature +V location 2 |
- | File: | + | File:BPA_+V_roomT_3.jpg|BPA room temperature +V location 3 |
- | File: | + | File:BPA_+V_roomT_4.jpg|BPA room temperature +V location 4 |
- | File: | + | File:BPA_noV_roomT_1.jpg|BPA room temperature no V location 1 |
- | File: | + | File:BPA_noV_roomT_2.jpg|BPA room temperature no V location 2 |
- | File: | + | File:BPA_noV_roomT_3.jpg|BPA room temperature no V location 3 |
- | File: | + | File:BPA_noV_roomT_4.jpg|BPA room temperature no V location 4 |
- | File: | + | File:BPA_+V_30_1.jpg|BPA 30 +V location 1 |
- | File: | + | File:BPA_+V_30_2.jpg|BPA 30 +V location 2 |
- | File: | + | File:BPA_+V_30_3.jpg|BPA 30 +V location 3 |
- | File: | + | File:BPA_+V_30_4.jpg|BPA 30 +V location 4 |
- | File: | + | File:BPA_noV_30_1.jpg|BPA 30 no V location 1 |
- | File: | + | File:BPA_noV_30_2.jpg|BPA 30 no V location 2 |
- | File: | + | File:BPA_noV_30_3.jpg|BPA 30 no V location 3 |
- | File: | + | File:BPA_noV_30_4.jpg|BPA 30 no V location 4 |
</gallery> | </gallery> | ||
Revision as of 19:35, 28 July 2011
Project |
July 24Started overnight cultures of pSB3K3 July 25PCR'd parts for pNT002: R0040, K123001, B0014, pSB4A5 ResultsDecided not to use the pNT003 PCR'd insert, as the band of the correct length was much fainter than a lower length band. Chose not to do Gibson assembly of the positive GFP control, despite not having any green colonies last week, instead focusing on assembling pNT002 and pNT003. PCR concentrations
PSB3K3 miniprep concentrations
July 26Packaging of 9mix and (10mix + 10-2) ligations Results
The gel gave no bands in any lane, with or without the restriction digest. pNT003 + without the Spe1 digest had a faint smear, the other lanes had nothing. Chose to transform anyway All enrichment cultures but one showed growth on LB.
July 27Grew up cells for titering of packaged fosmids; reached OD600 of 0.974 ResultsThe 16s PCR from yesterday, shown in the gel today, had a band in the first lane of negative controls, indicating contamination.
BPA solutions fail to form uniform layers when poured on top of agar, but form a uniform layer when poured directly onto a clean plate. July 28Plate packaged fosmids to obtain titer
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